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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 320 results for: POR C

Showing: 261 - 270 of 320 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal dynamics in response...leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors...

  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional...gene. Spatio-temporal expression of optogenetic reporters: Since most optogenetic reporters are delivered...invert the reporter and GeneR fragment. Then the second recombination event would excise the reporter from ...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ...Cre-dependent reporter expression, the potential for background expression of the reporter will be greatly...shown in Figure 2, the cassette should contain a reporter DNA sequence (such as GFP) in the sense orientation...the two pairs of target sites. By flanking the reporter with one loxP site and one lox511 site in the ...

  3. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...antibody structure with important features labeled. V = Variable domain. L = Light chain. C = Constant domain...from another portion. For example, Fab fragments are molecules composed of just the Fab portion of the antibody...between the target and reporter. They have fewer non-specific binding opportunities due to the lack of an...region is the “tail” of the antibody and comprises portions of the constant regions of the two heavy chains...used in vivo.     Of course, the lack of an Fc portion means that most of the common anti-IgG secondary...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

  4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses in the left and right...changes. In addition to supporting in-person conference attendance, the award now supports virtual conference...research.   Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...hydropersulfides modulate protein activity and have important roles during oxidative stress. To monitor this...

  5. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...manipulation. Common inbred strains include C57BL/6, Balb/c, and 129. Conversely, outbred strains were originally...gene incorporated into their genome. While transgenic mice have the new gene construct incorporated randomly...embryonic stem cells. Non-homologous recombination incorporates this DNA randomly into the mouse genome. In ...homologous recombination. A targeting vector is electroporated into embryonic stem cells and this will insert...vectors used to create knockout mice include a reporter gene, which replaces the targeted gene and provides...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...their monovalent nature. They are stable up to 80°C and can refold correctly after denaturing.  Designed...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

  8. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

  9. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...islands (regions of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to ... versions of this reporter to target the K9 (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27...The K9 reporter uses the HP1 chromodomain to recognise lysine 9 methylation, while the K27 reporter uses... methylation in living cells. Essentially this reporter construct consists of one substrate (the histone-derived...-K27 histone methylation reporter) positions on histone H3, which are known to be involved in repression...methylation of these sites was controlled, the reporter consistently showed significantly different FRET...states. Thus, this system is a reliable tool in reporting methylation status of these sites. This new technology...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... PubMed Central PMCID: PMC3622210. Schek N, Cooke C, Alwine JC. Definition of the upstream efficiency ...expression levels, the terminator also plays an important role in RNA processing and contributes to variability...polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, protection from degradation...degradation, and is integral to the nuclear export and translation processes as well.   Figure 2... non-poly(A) transcript-stabilizing and nuclear export elements such as WPRE (5) and CTE (6), and/or use...the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced HIV-1 Gag expression ...
Showing: 261 - 270 of 320 results