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...strand breaks (BLESS), revealed that these mutants do not cause off-target effects at unanticipated sites...

...application process? What types of writing feedback do you find most helpful? Have a great grant writing...

.... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ...

...Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit here!  Cloning...

...every C.elegans  neuron. Scientists are currently doing this using two approaches: single-cell RNA-sequencing...

...blurb you will use to introduce yourself and what you do. You might want to include your university and your...

... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...

...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...

...after knockdown of beta-catenin. Other gene targets do not require amputation to be visible. For example...

...  Listen to the CRY2clust podcast segment The Won Do Heo lab at the Korea Advanced Institute of Science...

...been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...

...backbone. Purpose Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses...

...Addgene.org Find Info on How to Quantify Your DNA Learn to Do a Bacterial Transformation Learn How to Perform a...

...study of heritable changes in gene expression that do not involve any changes to DNA sequence (Epigenetics...

...share your protocols with other groups. What apps do you use to improve your lab productivity? As this...

...plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past... are turned on or off in a given area and the can do so in a very specific, precise manner. Previously... are bound to the coding region are displaced and do not rebind because they are transported back to the...their quest to understand lncRNAs: What can lncRNAs do by themselves? The Rinn lab has developed a novel...

...referred to as “instillation” or “gavage,” as they do not require any punctures. Intratracheal instillation...

...right? Non-coding RNAs (ncRNAs) are transcribed but do not encode proteins. They play important roles in...

... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....

...01756-1. PMID: 37095348. Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022...