We narrowed to 617 results for: Dos

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

Learn how you can use RaPID to detect RNA-protein interactions and its applications in infectious and human genetic diseases.

Aug. 16, 2018, 12:40 p.m.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

Jan. 16, 2018, 3:20 p.m.

A paper published in Nature used CRISPR to correct a disease-causing mutation in human embryos. Does this paper herald a future of human germline editing?

Aug. 10, 2017, 2:19 p.m.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

May 9, 2017, 2:12 p.m.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

April 28, 2016, 2:30 p.m.

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Dec. 16, 2015, 3:30 p.m.

Five steps to help graduate students and postdocs write their first grant proposal.

Feb. 11, 2014, 12:13 p.m.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

May 6, 2025, 1:15 p.m.

Learn about the different open resources for C. elegans like OpenWorm, microPublication, and 3'UTRome, and find C. elegans tools at Addgene.

July 18, 2019, 12:55 p.m.

What you get out of a conference depends on what you put into it before, during, and after the meeting. Read on to find tips for honing your elevator pitch, the elevator pitch, navigating talks, networking, and social media.

May 23, 2019, 12:26 p.m.

Find tips and best practices for producing lentivirus in your lab: from choosing a packaging system to optimizing transfection and improving titers.

Aug. 7, 2018, 12:31 p.m.

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

June 7, 2018, 1:17 p.m.

Learn how you can use planarian plasmids from the Sánchez lab to teach developmental biology techniques to new molecular biologists.

May 24, 2018, 1:20 p.m.

Learn all about new optogentics tools, caffeine degradation, biosensors, and new fluorescent proteins.

May 15, 2018, 12:43 p.m.

Learn how and why AAV vectors are being used in cell and gene therapies.

Nov. 7, 2017, 1:59 p.m.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

March 8, 2017, 3:15 p.m.

In this blog post, learn about a new FRET-based biosensor for studying epigenetic modifications and the roles they play in inheritance/disease.

Nov. 19, 2015, 3:30 p.m.

Interested in sharing your lab protocols with others? Here are 7 software apps that can help you share and edit your protocols with other scientists.

Sept. 23, 2014, 3:05 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.