We narrowed to 623 results for: Dos
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TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....after knockdown of beta-catenin. Other gene targets do not require amputation to be visible. For example...
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Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m.... Listen to the CRY2clust podcast segment The Won Do Heo lab at the Korea Advanced Institute of Science... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...to be untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....every C.elegans neuron. Scientists are currently doing this using two approaches: single-cell RNA-sequencing... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....blurb you will use to introduce yourself and what you do. You might want to include your university and your... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m.... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....Addgene.org Find Info on How to Quantify Your DNA Learn to Do a Bacterial Transformation Learn How to Perform a... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....study of heritable changes in gene expression that do not involve any changes to DNA sequence (Epigenetics... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ... -
28 Hot Plasmid Technologies from 2015
TypeBlog PostPublishedDec. 23, 2015, 3:30 p.m....plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past... are turned on or off in a given area and the can do so in a very specific, precise manner. Previously... are bound to the coding region are displaced and do not rebind because they are transported back to the...their quest to understand lncRNAs: What can lncRNAs do by themselves? The Rinn lab has developed a novel...regulation has roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell ...reticulum (ER), mitochondria, cytoskeleton, and endosomes. These fusion proteins can act as markers for ...Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment mCherry-Rab5 BFP-Rab5 ... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....it is essential to make sure that the selected FPs do not influence each other and thus potentially falsify... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....electrical charges propagated through multiple cells to do things like translate perceived signals to physical... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, ... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....referred to as “instillation” or “gavage,” as they do not require any punctures. Intratracheal instillation... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....right? Non-coding RNAs (ncRNAs) are transcribed but do not encode proteins. They play important roles in... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m.... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more.... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....Cre-expressing neurons in a transgenic mouse line. By doing this, RABV expressing the viral protein EnvA on ... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....more about xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....Additional protein tagging systems For those who do not work with C. elegans, several groups have designed... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m.... used the library to identify RBD mutations which do not interfere with ACE2 binding, but impact recognition...