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...the chance of off-target effects, you still need to do your due diligence to ensure they are indeed a non-issue...off-targets don’t mess with your results when they do arise. Happy CRISPR’ing! References and Resources...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9...

... this given that the brain controls everything we do, but I was quite impressed by how neuroscientists...to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog Advanced...

...unbound) population. The benefit of beads is that they do not require cells from your experiment, and they ...prefers to use them to gate our populations, but others do not. Some journals require isotype controls, while...

...we have found ourselves adapting to a new way of doing things. These new processes have been instrumental... what we’re here for and we’re glad to be able to do so during this critical time. Visit our COVID-19 ...

...process is very similar for both, though tissue samples do require an extra homogenization step. For this blog...courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked...

...elute YFP, you turn on the blue lights. Which option do you choose?  Option #2 may sound like fiction, but...monomeric variable heavy chain antibody domain.  How do these components interact? When fused together to...

...similarity. REPLACR-mutagenesis: Letting bacteria do the work If you’ve conducted SDM using some of these...on in vitro assembly, they could make the E. coli do the work by using the principles of recombineering...

...? Blue-white selection is a widely used method to do just that! (To read about other screening strategies...with this method to ensure that positive colonies do have the plasmid containing the lethal gene. An example...

... all driven by personal connections What can you do to get the most from the time and money you are spending...about how you want to introduce yourself and what you do. You can probably use some scientific terms when ...

...unexplored areas with our color-changing flower, but to do that we’ve had to navigate the challenges involved... plant synthetic biology. We cannot build what we do not understand, and the complicated and unique metabolic...