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We narrowed to 772 results for: INA

Showing: 271 - 280 of 772 results

  1. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...with different varieties of tags, both N- and C-terminal. Each set of plasmids uses the same restriction... thorough introduction to pCri, please see the original publication cited below. pCri plasmids are available...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...

  2. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...Homologous recombination is the process by which nearly all domains of life repair genomic damage, specifically...their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized by specific... of yeast tagging modules with each possible combination of protease site (TEV or PreScission), epitope...protease site)-6xGly linker-(epitope tag)-ADH1 terminator-(selection marker). Additionally, Dr. Formosa...

  3. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...repress gene expression. In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read...editors with minimized off-target DNA editing The original cytosine base editor, BE3, induces a low frequency...validated CBEs with reduced Cas9-independent deamination. They generated these editors using the BE4max... Fusing epigenetic modifiers such as TET1 with inactive Cas enzymes can alter gene expression via acetylation...

  4. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ... centers in Boston.  I was relieved because it finally feels to me like the postdocs are taking some steps....  His list is dire:  The current system is unsustainable, hypercompetition is corroding values, we are...awards systems need to be radically overhauled.  Finally, the science publishing system needs to be changed... scientists to start addressing this problem.  Finally, one point made by Dr. Graham Walker, Professor...

  5. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ... the discussion in markers on a whiteboard. The final image is pictured here…she started from a blank ...labs and the importance of the strong, cross-disciplinary collaboration they shared to the work they did...extra fun) who shared first authorship on their seminal, cooperative paper in Science.  Interestingly, ...forever. He emphasized the potential of this extraordinary breakthrough. In fact, the panelists agreed ...

  6. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ... nuclease folds found in other proteins. The C-terminal domain (CTD) of the protein also belongs to the...Sternberg et al., 2014). The nuclease lobes are inactive in the absence of guide RNA, preventing Cas9 from...loaded and the DNA sequence is recognized, it’s finally time to cut! The HNH domain, which cuts the target...flexible; it can rotate about 140 degrees between inactive and active conformations (Bravo et al., 2022; ...on this topic are still being made! Conclusion Finally, we can summarize the process from start to finish...

  7. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...the AAV genome’s two inverted terminal repeats (ITRs); but when recombinant AAV (rAAV) is made in the lab...generates the different C-terminal regions of the Rep proteins. While the unique N-terminal domain of Rep78 and...

  8. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...sequence through a mechanism called depurination. In depurination, the hydrolysis of the glycosidic bond... DNA stability in solution are pH and DNase contamination.  Extreme pH can denature, break, and even change...gene, there are usually extra steps like heat inactivation, protein degradation, and/or the use of chelating...

  9. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...still free, and different regions of the spinal cord were illuminated. By looking at groups of neurons that...expressed the LiGluR opsin in different subsets of spinal cord neurons using the Gal4/UAS system. This created...dissection of a behavioural module in the vertebrate spinal cord." Nature 461.7262 (2009): 407. PubMed PMID...

  10. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...lines: ones either suspected to be contaminate or easily contaminated, as well as commonly misidentified...informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to access Cellosaurus...profiling, you can use Cellosaurus, and CLASTR, to finally know whether it was an 'h' or a 'b' written on ...
Showing: 271 - 280 of 772 results