...For the last 14 years, scientists have been coming to the Addgene website in search of plasmids. Now, scientists...synthetic biology page Search for a plasmid Find Addgene's most popular plasmids ...Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing...at its sequence. MIT collaboration for gene classification This inquiry started when Neil Thompson, a ...is synthetic or natural with 97.7% accuracy. Classifying genes as natural or synthetic But how can you...organism, it may express protein more efficiently. To classify gene sequences as natural or synthetic, the researchers...simulations and machine learning, they created a classifier that predicts whether a gene was natural or synthetic...

....org Find Cre-Lox Plasmids Find Optogenetics Plasmids Find Chemogenetics Plasmids ...vectors expressing light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience.... These Cre/ FLP versions are retained in the cytoplasm until estrogen receptor ligand Tamoxifen is added...neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and the...recombinase. After in-utero electroporation, this plasmid mix results in sparse labeling of cortical neurons...labeling of individual neurons one could reduce the plasmids amounts to reduce transfection efficiency. However...visualized at the same time from two different plasmids this approach becomes problematic as it reduces...

...single plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...pooled library, which contains a collection of plasmids in a single tube, or a pre-made viral prep library... prepare the libraries for you.  Starting from plasmids requires amplification, verification, and lentiviral...library and appropriate packaging and envelope plasmids into HEK cells allows production of lentivirus...amplify the library. This is similar to amplifying a plasmid: transform E. coli with the pooled library DNA,...own protocol that amplifies without disrupting plasmid representation. A good place to start is with a...

...that the plasmid could be maintained in the absence of antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe... 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent well of HEK... Fig. 1: Expressing bARGSer in E. coli. (a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b) Methods to ...overexpressed compared to accessory proteins. Excess gvpA plasmid added into the transfection mixture in HEK293T ...mammalian species to produce gas vesicles. New mARG plasmids Our new mARGs come as a set of two PiggyBac integratable...genes gvpNJKFGWV marked with Emerald GFP. These plasmids can be used directly in transient transfection... into the genome by cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines...

...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...work to share plasmids to all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse...Biological repositories that distribute mouse strains, plasmids, cell lines, and much more have been critical ...science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, 2020 to ...to July 27, 2020. Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents...

... After all, students build circuits in physics class to learn about electronics or mix chemicals together...everyone safe. None of this is conducive to a classroom setting, especially for a school with a limited... activities making their way into high school classrooms, like gel electrophoresis to separate DNA or ...translation. Unfortunately, having live cells in a classroom presents equipment needs, such as shakers and ...FD-CF pellets are easy for students to use in the classroom because the students simply need to add water ...developed the BioBits kit to be used in high school classrooms, but it could be widely adapted and used in a...transition your undergraduate students from the classroom to the lab. You could give your undergrads hands-on...

...Read More: All Plasmids 101 Articles Browse plasmids that contain luciferase ...Luciferases are a class of enzymes capable of catalyzing chemical reactions in living organisms resulting...choose from) is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert ...chemical reaction compared to using a high-intensity laser to rapidly excite GFP. As a result of the different...hirtus) 630 64 Luciferin ATP No Common uses for plasmids expressing luciferase Luciferase reporter assay...assay The typical luciferase-containing plasmid is most often used to investigate the effect of regulatory... cloned upstream of the luciferase gene in the plasmid, which is then introduced into the desired mammalian...

...Collection page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for...microbial research. Kits, guides, plasmids, and more We have Plasmid Kits for microbial work with fluorescent... To make it easier for you to find plasmids for your work, plasmids in the Microbiology Collection are... a useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally...us at [email protected]. If you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene...develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org/deposit ...

...transfection of single plasmids vs pooled plasmids; for multiple edits, single plasmids are generally more...transfected on a single plasmid. This is versus 2.4% of cells transfected with a pool of plasmids that contained...sites. This eliminates bulk from crRNA expression plasmids resulting in smaller, more streamlined vectors...contained one crRNA per plasmid plus a Cpf1 expression vector.  See the graph in figure 2 for a comparison of...of a crRNA array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes...your research? Check out the Cpf1 multiplexing plasmids available from Addgene. If you have any questions.... Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell,163(3), 759-771. PubMed...

... expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression vector... express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing... (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based ...been engineered to perform unique editing tasks, classic Cas9 nuclease, which introduces double stranded...lentiviruses. This means that if you received lentiCas9-blast lentivirus from Addgene, you could use it in conjunction...own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing...with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes...