Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 320 results for: POR C

Showing: 271 - 280 of 320 results

  1. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which can be used to confirm...Professor at Boston College. National reports stress the importance of providing authentic research experiences... is particularly interested in strategies to incorporate research into undergraduate courses and in communicating...

  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... Cas9 to generate the mutant cells, respectively (C). Mutant cells are screened (D) and "hits" are identified...post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...not entirely known. Identifying which genes are important for a phenotype can lead to a wealth of additional...strong evidence that a particular gene is actually important for a given phenotype. Each hit from the screen...

  3. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...//doi.org/10.1016/s0091-679x(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...utilizes Cas9 proteins harboring receptor ligands (C in the figure above), which result in the internalization...delivery of RNPs is electroporation (A in the figure above), which generates pores in the cell membrane...and gRNA expression       Plasmid DNA may be incorporated into the genome     Off-target effects can ...through a process known as CRISPR-EZ (CRISPR RNP Electroporation of Zygotes) (Chen et al. 2016). For CRISPR ...CRISPR experiments that involve HDR, electroporation can be combined with cell-type specific reagents in a ...a technique known as nucleofection, which forms pores in the nuclear membrane, allowing for entry of a... account with these types of experiments. One important consideration is that the Cas9-gRNA RNP will be...

  5. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...media, reducing the incubation temperature (e.g., 30 °C), and limiting your growth time (e.g., 16-18 hours...individual libraries can vary significantly, it’s important that we have an amplification protocol developed...and the desired coverage for the library (How important is it that every single plasmid in your library...protocol. Created with BioRender.com.   The most important factor to consider is your desired library coverage...not millions, of plasmids, they are generally electroporated into electrocompetent cells to ensure the highest...chemical transformations is less than that of electroporation, but an advantage is that chemical transformations...protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...

  6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...the most common uses, GFP can be fused to the N- or C-terminus of a protein, which allows the scientist ...a fluorescent emission wavelength in the green portion of the visible spectrum (hence the name), which...maintaining fluorescence. The protein structure, first reported in 1996, is an eleven β-sheet-containing “barrel...solvent. This tightly-packed structure explains the importance of the entire GFP protein, which is almost completely...constructing fluorescent fusions. Transcription Reporter: Placing GFP under the control of a promoter of...a given cell type. This type of transcription reporting was among the earliest uses of GFP. Fluorescence...protein-protein interactions. In this case, two portions of EGFP are fused to the proteins of interest,...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... targeting mCherry reporter (mChe), with co-incubation with assist peptide (AP). C) Workflow for Cas-RNP-PAGE...difficult to genetically edit due to toxicity from electroporation or poor cellular entry with other transfection.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized by pre-existing antibodies...

  8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber...pair to another base pair at a specific site. 2. Temporal, spatial, or conditional control of CRISPR activity... way to make those genome edits. But, just as important is a way to flip the switch and turn off CRISPR...The ability to turn off CRISPR activity is an important step towards CRISPR-based therapies and for more...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

  10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS One. 2011...have that conversation out in the open is really important. Addgene: Is there anything else you’d like to...biology is massively underfunded considering how important it is. I would really like to reach all those ...
Showing: 271 - 280 of 320 results