We narrowed to 884 results for: ica

...The fix/perm solutions can make a significant difference in the efficacy of your antibody. When compared...procedure enables quantification of antibody performance in major research applications. eLife, 12, RP91645...that the antibody being published doesn’t always indicate the presence of supportive data. To be supportive...that the antigen being detected in the intended application — here, flow cytometry — is the desired target... saponin. 400,000 cells were stained with the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb...multiple timepoints. When the target of interest is critical to cell survival or proliferation, an additional...this approach should also allow for the clear identification of the cell types of interest. By correlating...

...drives and their ramifications. Readers unfamiliar with academia’s challenges with publication and funding ...and CRISPR of course address some of the ethical implications of using these technologies, but also the...Connecticut College who has been teaching and communicating science for over two decades, attempts to answer... primarily on fluorescent proteins and their applications, tackles much broader issues in this brief volume...sexism in science, problems with peer-reviewed publications and funding, bioethics in the age of CRISPR,... and her essential-but-unlauded role in the identification of DNA’s double helix. Perhaps to the dismay...dismay of middle-aged scientists, the identification of Green Fluorescent Protein (GFP) and its development...

...therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section. In order ...and aceylation, respectively (these are chemical modifications, or functional groups, found on lysine)..., start, and stop. For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the ... Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones, a genomic region...(ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG in the human genome. ...maintaining ESCs. So which of these 13,000 regions are critical? One hypothesis: the enhancers! This leads us ...approach for designing an experiment to identify the critical NANOG binding sites: 1. Find Enhancers that have...

...containing a phage origin of replication that can be packaged into replication-incompetent phage particles...bacterial host range. To further potential clinical applications, Ando et al. recently engineered synthetic...the bacterial population. But what if we could specifically target only virulent or antibiotic resistant...essential processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target such...antibiotics by their suppliers, and public health and medical organizations are working to help educate the public...encoded by a high-copy plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single...represented here by cells of different colors, specifically targets bacteria with the given genomic or plasmid...

...networks in biological systems. NGS will also play significantly into the realm of clinical therapeutic...detection of KRAS mutations: diagnostic and clinical implications." The Journal of Molecular Diagnostics 12.4...validation process, also known as CRISPR genotyping, is critical to demonstrating causal relationships between ... Figure 1: Schematic representations of the indicated unbiased genotyping options. Figure reproduced...those relating to DNA structure (e.g. epigenetic modification, bulges) that may also impact off-target edits...cell-based methods are genome-wide, unbiased identification of double-strand breaks (DSBs) evaluated by... amplicon sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer ...

... We’ve also created constructs with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-...animals and fix the samples. When you use AAVs, it typically takes a few weeks to achieve optimal expression... Any longer and the cells may start to show morphological abnormalities due to the excessive amount of... levels of XFPs - Reduce the amount of tTA. Paradoxically, too much tTA leads to a reduced expression ...most likely by suppressing transcription. It is critical to express a minimal amount of tTA to achieve ...sensory systems focusing on the functional and anatomical maturation of the olfactory system.      Takeshi...University. His lab has been studying functional and anatomical aspects of sensory circuit development using ...

...basics but are here because you want to know the intricacies of transductions, we've got you covered there...The good news is that viruses are very valuable biological tools, and with the right safety considerations...infiltration into a cell. The twist is that the typical genetic cargo — the viral genome — is swapped, ...vector containing the cargo of interest is then typically transfected along with viral packaging plasmids...risk management when working with viral vectors. Typically, one day after transfection into the production...expression or integration resulting from transduction typically plateaus within a few days to a few weeks, depending...viral preps available for a variety of broadly applicable and popular viral vectors. Check out our viral...

...Effective verbal and written communication skills are key to publication and acquisition of funding. If...  Writing Communication Publicity Consulting internships Communication Teamwork Management...Models Communication Regulatory knowledge Networking Blogging Writing Communication Networking... skills such as leadership, management, and communication. A great approach to fully understand and acquire...find open positions and view their required qualifications and skill sets Taking the time to identify... science class: Science Club for Girls) Communication to diverse audiences Organization Mentorship...  Leadership Management Marketing Communication Business models Conference or club organizer...

...has and will continue to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR... D1135E variant is far more selective for the canonical S. pyogenes PAM sequence NGG compared to wild-...variant may increase the specificity of genome modifications at DNA targets adjacent to NGG PAM sequences...VRER variants is similar to wild-type SpCas9, indicating that the variants are likely just as selective...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...doi.org/10.1038/nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo T, Park SW, Kim D, Kim...10.1038/ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver BP, Prew MS, Tsai SQ...

...then separated by size using electrophoresis, typically capillary electrophoresis. Capillary electrophoresis...sample at a time, which can become costly and impractical for whole-genome (and often, whole-plasmid) sequencing...many samples and can output more data, and it typically results in high accuracy for full plasmids. However...attached to the flow cell membrane and have an electrical current running through them. DNA or RNA is pulled...each nucleotide passes through the nanopore, the electrical current recorded by the instrument changes. Base-calling...sequences submitted by depositors. A sample will automatically pass through our QC process if there is a 100%...for discrepancies in backbone sequences. Most theoretical sequences submitted to Addgene have minor differences...