We narrowed to 841 results for: Abo;

...applications and there’s just not much to be done about that.  Second, expansion introduces distortions....

...Microbiology, National Emerging Infectious Disease Laboratories.  She is interested in genome replication and...

...University of Tokyo, Osaka University). Can you speak about your different experiences in research labs. What...

.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

... also toxic at high concentrations as described above and is inactivated by the complement system in serum...

...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar ...

...Collection and the BRAIN Armamentarium Collection, a collaborative effort that the Allen Institute contributes ...

...precise and accessible genome engineering. More elaborate protocols are available in our earlier publications...

...sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...

...conjugate antibodies themselves, the process can be laborious and expensive. In some cases, conjugation methods...

...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

...ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus. But pre-made...

... Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

...plasmids may have high copy numbers and reduced metabolic load (Meyer 2009). Figure 1: Idealized ...

...you enjoy playing with our genes. Have fun!    About the authors: Marjorie Buss is a PhD student studying...

... & Corey, D. R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research...

...transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab...

...limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...

... Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits ...

... Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to...

...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...

...which tools to use, and what do you need to know about them to be successful in your experiments? In this...

...starting the selection process. Factors to think about are (1) your experimental conditions and measurements...

...to label the rat brain sample shown in Figure 2A above, Andrews et al. used the following combination of...

...our planet, but we still know collectively little about how they infect and influence the evolution of their...

...target should be near the TSS but you can worry less about sequence optimality because you generally have fewer...

... A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020...

...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

... should have a melting temperature 5-10 degrees above the primer and be at least 25 bp away from each ...

...If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices...