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...right? Non-coding RNAs (ncRNAs) are transcribed but do not encode proteins. They play important roles in...

... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....

...01756-1. PMID: 37095348. Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered...

...electrical charges propagated through multiple cells to do things like translate perceived signals to physical...

...Additional protein tagging systems For those who do not work with C. elegans, several groups have designed...

...it is essential to make sure that the selected FPs do not influence each other and thus potentially falsify...

...views in this post are those of the guest blogger and do not necessarily represent the views of Addgene. Addgene...

...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

... GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You...

... pooled libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled...

...your sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing...

... spin columns you need to complete your research. Do you know of other nucleic acid purification methods...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

...in the biosynthetic pathway. Why plant scientists do transient leaf transformations The development of...

...Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger or TAL proteins...

...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...

...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...