We narrowed to 629 results for: Dos

...Cre-expressing neurons in a transgenic mouse line. By doing this, RABV expressing the viral protein EnvA on ...

...more about xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there...

...Additional protein tagging systems For those who do not work with C. elegans, several groups have designed...

...Integrative and conjugative elements (ICEs): what they do and how they work." Annual review of genetics 49 ...

...ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, ...

...it is essential to make sure that the selected FPs do not influence each other and thus potentially falsify...

...views in this post are those of the guest blogger and do not necessarily represent the views of Addgene. Addgene...

...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

... become valuable tools for your research! If they do (or if they don't!), please consider sharing your...

..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

... GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You...

... pooled libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled...

... spin columns you need to complete your research. Do you know of other nucleic acid purification methods...

...your sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...