We narrowed to 617 results for: Dos

Learn about the most common injection routes in mice, including their key differences and uses.

Jan. 22, 2025, 9:42 p.m.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Nov. 14, 2024, 2:15 p.m.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!

Aug. 29, 2024, 1:15 p.m.

Summary of degron tags along with their applications, advantages, and several commonly used tags.

March 14, 2024, 1:15 p.m.

In our latest Hot Plasmids, we feature new systemic capsids, Cas-Page, an anti-FLAG antibody, and more!

Feb. 20, 2024, 2:45 p.m.

A review of the types of voltage indicators available for use as well as how viral vectors aid in the implementation of these systems.

Jan. 30, 2024, 2:15 p.m.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

May 18, 2021, 1:15 p.m.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

July 23, 2020, 1:20 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.

Learn how rabies virus can be used to trace neuronal connections. AAV available at Addgene can help you with the process!

May 29, 2018, 1:51 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.

Get tips on how to monitor your protein of interest inside cells - co-localization, complex formation, and more.

June 22, 2017, 2:30 p.m.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Aug. 4, 2016, 2:30 p.m.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

May 26, 2016, 2:30 p.m.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

May 15, 2014, 3:33 p.m.

Addgene answers questions about lentiviral plasmids, including 2nd vs 3rd generation transfer vectors, packaging plasmids, biosafety concerns, and more.

April 23, 2014, 1:08 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

March 11, 2025, 1:15 p.m.

Addgene's antibody lab member Kate describes each step in recombinant antibody production process.

Aug. 13, 2024, 1:15 p.m.

Ready to produce rAAV in your own lab? This 101 post can help!

July 16, 2024, 1:15 p.m.