We narrowed to 772 results for: INA
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TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....some public speaking and a bit of video work for webinars (get tips for your next science talk). I do illustrations...website quite a bit.” Out of all of these tasks, webinars are Hans’ favorite thing to work on. “It’s one...someone to take over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am...getting abstracts and plans together for my next 4 webinars—I have them stacked close together for the next...
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Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....guide to becoming a successful writer. When I originally talked to my PI about becoming a better writer...aspects to it: videos starring Roberto Kolter, webinars taught by Peggy Cotter, Mary O’Riordan, and Victor...don’ts of that week’s piece of a manuscript. The webinars were my favorite part due to the interaction with... the individual figures and results to make the final product with an interesting introduction and concise... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....Ultragenyx Pharmaceutical in 2017. As one of the original employees at Dimension Therapeutics, she helped...interested in for a long time. “At an early age, I was fascinated by the impact that medicines can have on human...during graduate school at MIT. “I read some of the seminal papers on cell cycle discovery from Lee Hartwell...delivery. In 2018, he interviewed Addgene co-founder Melina Fan and former Associate Director of Viral Vectors... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....preparation approaches described previously require a preliminary double stranding step. The double stranding step... library preparation procedures. This finding eliminates the double-stranding step and reduces both the...confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an open-source...uses the VGS data to determine the percent of recombination present in Cre-dependent vectors and a script... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m....electrical activity through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used...Chimeric channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363 AAV5 AAV ...labeled neurons because background fluorescence from inactive neurons is reduced. 105322 AAV1 pGP-AAV-...densely-labeled neurons because background fluorescence from inactive neurons is reduced. Additional resources... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....disturbing the tissue for imaging or collection could contaminate it. I have used it for selecting transformation...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al....untransformed seed from the same plant beginning to germinate. (Top right, second panel) Transformed seedlings... Biology Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....the first vaccination and after both vaccinations. For the B.1.351 variant, a single vaccination didn’t ... SARS-CoV-2 variants coincide with increased vaccination efforts. So, how effective are the current vaccines... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....particles. A single viral particle is called a virion. Finally, viral vectors, which are used in the lab, are ...contrast to bacteria and eukaryotes, which use a binary replication process whereby one cell copies itself...engineering controls — changes made to the virus that eliminate or reduce the risk of infection — researchers ...genetically modified to either limit or completely eliminate their replicative ability. The remaining particle... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....sequences on a daily basis, many found these tools inadequate for planning, visualizing, and documenting their...elements such as promoters and transcription terminators, the boundaries are less obvious. We found that.... In an effort to be rigorous, we dug into the original literature, some of it decades old, to provide...mismatches, our algorithm could annotate a feature inaccurately. The best example is fluorescent proteins, which... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....next experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic...inputs are received by a cell. These so-called recombinase-based state machines could potentially be used...development. Read this post to learn more about recombinase-based state machines and how they function. Adapting...them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that...