We narrowed to 783 results for: INA

...repress gene expression. In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read...editors with minimized off-target DNA editing The original cytosine base editor, BE3, induces a low frequency...validated CBEs with reduced Cas9-independent deamination. They generated these editors using the BE4max... Fusing epigenetic modifiers such as TET1 with inactive Cas enzymes can alter gene expression via acetylation...

... centers in Boston.  I was relieved because it finally feels to me like the postdocs are taking some steps....  His list is dire:  The current system is unsustainable, hypercompetition is corroding values, we are...awards systems need to be radically overhauled.  Finally, the science publishing system needs to be changed... scientists to start addressing this problem.  Finally, one point made by Dr. Graham Walker, Professor...

... the discussion in markers on a whiteboard. The final image is pictured here…she started from a blank ...labs and the importance of the strong, cross-disciplinary collaboration they shared to the work they did...extra fun) who shared first authorship on their seminal, cooperative paper in Science.  Interestingly, ...forever. He emphasized the potential of this extraordinary breakthrough. In fact, the panelists agreed ...

... nuclease folds found in other proteins. The C-terminal domain (CTD) of the protein also belongs to the...Sternberg et al., 2014). The nuclease lobes are inactive in the absence of guide RNA, preventing Cas9 from...loaded and the DNA sequence is recognized, it’s finally time to cut! The HNH domain, which cuts the target...flexible; it can rotate about 140 degrees between inactive and active conformations (Bravo et al., 2022; ...on this topic are still being made! Conclusion Finally, we can summarize the process from start to finish...

...lines: ones either suspected to be contaminate or easily contaminated, as well as commonly misidentified...informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to access Cellosaurus...profiling, you can use Cellosaurus, and CLASTR, to finally know whether it was an 'h' or a 'b' written on ...

...guide to becoming a successful writer. When I originally talked to my PI about becoming a better writer...aspects to it: videos starring Roberto Kolter, webinars taught by Peggy Cotter, Mary O’Riordan, and Victor...don’ts of that week’s piece of a manuscript. The webinars were my favorite part due to the interaction with... the individual figures and results to make the final product with an interesting introduction and concise...

...sequence through a mechanism called depurination. In depurination, the hydrolysis of the glycosidic bond... DNA stability in solution are pH and DNase contamination.  Extreme pH can denature, break, and even change...gene, there are usually extra steps like heat inactivation, protein degradation, and/or the use of chelating...

...still free, and different regions of the spinal cord were illuminated. By looking at groups of neurons that...expressed the LiGluR opsin in different subsets of spinal cord neurons using the Gal4/UAS system. This created...dissection of a behavioural module in the vertebrate spinal cord." Nature 461.7262 (2009): 407. PubMed PMID...

...the AAV genome’s two inverted terminal repeats (ITRs); but when recombinant AAV (rAAV) is made in the lab...generates the different C-terminal regions of the Rep proteins. While the unique N-terminal domain of Rep78 and...

...some public speaking and a bit of video work for webinars (get tips for your next science talk). I do illustrations...website quite a bit.” Out of all of these tasks, webinars are Hans’ favorite thing to work on. “It’s one...someone to take over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am...getting abstracts and plans together for my next 4 webinars—I have them stacked close together for the next...