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We narrowed to 320 results for: POR C

Showing: 281 - 290 of 320 results

  1. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...4. Mittereder, Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation of the concentration and bioactivity... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can...

  2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...due to camelid high body temperature (37.2 - 39.2°C). The Seeger lab found a way around this by using ... Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody...

  3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ... where they could bind to intracellular epitopes. C) Permeabilizing with a non-ionic detergent, like Triton... for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with the selective...

  4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol. 2021 Yujie Sun   For even...of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J. 2010... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo....  Controls  When designing experiments, it’s important to include proper controls to assess the behavior...target proteins, especially if the termini are important for their interaction. You can read our previous...

  5. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...AAV11 is reported to target skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent... available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color compatible with the...and AAV11 tropism and applications. A) AAV6 is reported to have broad tissue tropism, including skeletal...poses challenges, from non-selective anterograde transport to a lack of genetic control. ATLAS (anterograde...the Cre recombinase payload activates a floxed reporter gene (genetically encoded or delivered separately...

  6. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...20205762. PubMed Central PMCID: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...), the same cloning procedure can be used to incorporate each element into the final construct. This strategy...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...sequences), so enzyme choice is critical. May not support in-frame fusions of coding sequences Sladitschek...

  7. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...Moreno-Mateos, M. A., Fernandez, J. P., Rouet, R., Vejnar, C. E., et al. CRISPR-Cpf1 mediates efficient homology-directed...typically require an exact DNA sequence to be incorporated at a precise genomic location, with little to...template for repair, which will result in the incorporation of your sequence, a.k.a a knock-in! So how does...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...molecule is used as the template, you need to incorporate homologous sequence to the right and/or left ...

  8. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...be used for promoter swapping and generating N and C terminally tagged fusion proteins with fluorescent...analyzable transgenic in hand, so it is pretty important to get this process right the first time. I’ll...to transform when they begin flowering. It is important to perform the transformation not long after flowering...cut off the primary inflorescence (the flowering portion of the plant) near the base of the stem. This buys...s time to select for transformants. It’s very important to select multiple T1 plants from each transformation...

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ..., Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...reducing the chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik...species, or even an antibiotic resistance gene. Importantly, native, non-pathogenic bacteria remain to help...colonization model, phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just...
Showing: 281 - 290 of 320 results