We narrowed to 610 results for: ACE;

1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future of the...

2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ... a Cas9 mutant (Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing...

3. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...that you can bring into pretty much any other workplace. To get your mind working, take a look at the ...

4. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...assembled next to each other, a third module cannot be placed between them, but rather, can only be added to ...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...of your PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such...

6. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   Published

   Sept. 6, 2022, 1:15 p.m.

   ... or if and how much the spread of the virus to adjacent areas impacted the experiments we wanted to perform...

7. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...offers is marginal. Fortunately, amp can easily be replaced with carb if satellite colonies are an issue. ...

8. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   Published

   Feb. 9, 2023, 2:15 p.m.

   ....     Fig. 2: Students in an MDH CURE replaced select residues of human mitochondrial MDH (hMDH2...

9. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ...Galacteros F, Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467...

10. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...verify if the colony PCR primers work in the first place: the backbone vector with and without an insert....

11. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...transcription (Figure 1). All viral genes are removed and replaced by a single (non-viral) reporter gene (e.g. firefly...

12. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...yeast! Many false negatives stem from or are exacerbated by expression of target proteins in heterologous...

13. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...Cas9’s, but with the direct repeat preceding the spacer rather than following it. The Cpf1 crRNA is also...

14. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    Published

    Aug. 20, 2015, 2:30 p.m.

    ...different issues, and our program didn’t stay in one place within the city infrastructure. My career path changed...

15. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...'ve found that research institutes in different places have very different cultures. In Basel, I felt ...

16. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    Published

    April 23, 2014, 1:08 p.m.

    ...expression of not only the transgene but also that of adjacent cellular genes. Q7: Which genes are deleted or...

17. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...“in the zone” and everything seems to fall into place. When the balance tips, we need to look to for support...

18. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...all genes involved in the production of a cell surface glycan that’s required for ricin uptake. The ricin...

19. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...social media is a connector and virtual meeting place: You can follow interesting people on twitter and...

20. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ... 21243013. PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal T...