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  2. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody...

  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember...

  4. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image...

  5. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...

  7. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of...

  10. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ...

  11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ... for non-neural cells, ion-based communication happens at a slightly slower scale.  All living cells ...After attending this meeting I had an increased appreciation for the role of ion channels and pumps in developmental...

  12. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...

  13. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this...

  14. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...physicist, etc., is an immense task (it takes, ooh, approximately the length of your average PhD to achieve!) ...not communicating as clearly as you intended (it happens to us all!) and offer a variety of options to make...

  15. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

  16. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...antibody you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing...

  17. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins...

  18. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail, diseases and...Fragile X syndrome, etc.). Hopefully now you can appreciate double stranded isn’t just for DNA. Untwisting...

  19. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

  20. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...Addgene is a totally invaluable resource. I really appreciate the work you do to make sharing reagents and ...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...
Showing: 561 - 580 of 801 results