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Showing: 561 - 580 of 814 results
  1. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...
  2. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...
  3. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image...
  4. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...
  5. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember...
  6. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...
  7. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...
  8. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of...
  9. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ...
  10. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...Addgene is a totally invaluable resource. I really appreciate the work you do to make sharing reagents and ...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...
Showing: 561 - 580 of 814 results