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Showing: 561 - 580 of 780 results

  1. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...techniques are continually being refined; it is completely plausible that scientists will eventually have...standardized process of good research practice: computer simulations, in vitro research, animal models,...

  2. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters such as...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

  4. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...custom primers or "primer-walking" reactions are completely unnecessary for sequencing multi-kb DNA inserts...seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

  5. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...playing a card game called “Magic”. There were complicated rules that governed how this game was played....should be “brains-on”, but as much as I try being compelling, interactive, and application-driven to boost...

  6. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...   Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head for gene expression changes of non-target...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...

  7. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

  8. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Musicophilia is accessible and fascinating, all without compromising on the relevant science."     - Randall Carter...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...

  11. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ... creates a non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

  12. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...increase in the number of endogenous indel edits when compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA...expression. As a proof of concept the Denham lab compared editing at three different genomic sites in the...

  14. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...

  15. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...

  16. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

  17. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate...add methyl groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...lentiviruses expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs...expression between experiments and over time (as compared to separate transductions with a single-vector...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...
Showing: 561 - 580 of 780 results