We narrowed to 783 results for: INA

... DNA that you want to assemble together in a combination of different ways according to your cloning strategy... or PCR products from a gel. You will avoid contamination from other DNA fragments and you will remove...

...plasmid copy number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing...multi-protein complex, stabilized promoters can eliminate variability between the different components. ...

... during, and after organizing such a workshop. Finally, the group put a lot of effort into ensuring that...them can do more harm than good. Check out this webinar for more on this. Be on the lookout for upcoming...

...expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective... Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also a quantitative...

... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...when designing your primers can be found here. Finally, you will want to have a well-organized database...

...combine the best of these mutations and tested the combination mutants for their on-target and off-target activity...that eSpCas9(1.1) and SpCas9-HF1 each stall in an inactive form after binding to a target site and propose...

...was contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog ...In others, the experiments are geared towards eliminating possibilities until the results focus in on a...

...complexity. We have characterized almost 600 terminators. That gives us enough hardware to control hundreds...over the next year. Then there will both be a combination of parts in Addgene along with the software to...

...carrying through on the actions you committed to. Finally, hiring a paid coach will make you take the experience...sensitive to this and have coaching plans that are financially accessible. Sometimes only a few hours of coaching...

...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...immune system of bacteria. These Acr proteins were originally found in type I CRISPR-Cas systems.   Four years...

...conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP is added to the wells. This... the amount of your protein of interest in the original sample. The competitive ELISA is best used for...

... DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent protein...Ward lab created a tool-kit to expand upon these original tools that allows for both spatiotemporal protein...

...participate in a science photo competition. Or nominate yourself as an educator for a short, animated ...Masterclass in science communication. The National Co-Ordinating Centre for Public Engagement (NCCPE) provides...

...enable us to measure these properties, and what fascinating experiments could push these tools even further... Förster Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation...

... One way to triumph over your neighbors is to eliminate the competition, and Penicillium isn’t the only...exciting molecular technologies!  This post was originally written by Jessica Welch in January 2017 and ...

...reference organisms with the overexpression plasmids. Finally, students determine if the presence of the plasmid...Government Office of Science and Technology. 3. Duina, A. A., Miller, M.E., & Keeney, J.B. Budding yeast...

... determine the best solutions. The event also culminated in a joint American Society for Biochemistry ...Officer Harvard University  The symposium will culminate with a keynote address by former congressman and...

...applications. Get More Cpf1 Background Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN ... What we still don't know about Cpf1 In their original paper characterizing Cpf1, Zetsche et al. suggested...

...increase the enrichment of target-specific sybodies. Final enrichment via enzyme-linked immunosorbent assay...each sybody candidate. These tags are used in a final enrichment step for identifying sybodies that bind...

...exchange occurs through two sequential homologous recombination events: The plasmid carrying the desired genetic...activity. ShadowR can be used as a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa ...