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Showing: 561 - 580 of 758 results

  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...their academic science careers in order to head to Ireland and take a chance as entrepreneurs. To make things...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...

  2. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,..., M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    .... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using...emission and absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers...

  6. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...in the E. coli lab strain DH5a, but others may require strains such as ccdB survival (for Gateway plasmids...

  7. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...poster: those that want you to walk them through the entire poster, or those that want to look through themselves...handle on your nametag for the tweep up (and for the entire conference, in general). After the conference Review...

  8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ..., the drive could eventually spread through the entire wild population with unpredictable ecological effects...ecological, and reproductive confinement typically require very little effort to implement (depending on the...

  9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

  11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...think of as an operating system for engineering an entire cell. Typically when people use plasmids, they ...much easier. So you could imagine engineering the entirety of a cell. That's a big long-term technical challenge...

  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...choose, you may not be able to elute the antibodies directly into your preferred buffer. If that’s the case...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

  13. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...impacted the experiments we wanted to perform. It required a great deal of time and effort to test and optimize...J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives...

  14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...availability following cross-linking fixation; they require an additional step generally referred to as Antigen...retrieval here because it’s not quite as ubiquitously required for IF as fixing and permeabilizing.  References...

  15. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets endogenous...

  16. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular...

  18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...modeling isn’t up your alley, feel free to skip directly to the results. To start modeling our data, we...chemistry and chemical biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the ...

  19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ... that if an essential gene plays a role in the desired screening phenotype, it may be missed because it's...transcriptional machinery to dCas9 and its RNA directed binding site. These methods include the fusion...

  20. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...affinity purification, in 2009. However, iChIP requires insertion of recognition sequences of an exogenous...into the target region. However, it struck me that direct use of such engineered DNA-binding molecules even...
Showing: 561 - 580 of 758 results