We narrowed to 629 results for: Ncl
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TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....all of the differentiated cells in an organism, including the embryonic and extraembryonic cell types. In...
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Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....… A no template control (NTC) should always be included. While the physical titer generated by qPCR is... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....mind that there are many other cloning methods - including some that rely solely on PCR, and a host of commercially... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....ll be brought under the hood with 70% ethanol, including your glove-covered hands! You should additionally... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m.... xCas9 2.0-2.6. This second round of evolution included continuous outflow of phage, increasing stringency... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....Cancer Research Annual Meeting, you might consider including more background on how your virus of interest ... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....Many companies have industry postdoc positions, including AstraZeneca, Pfizer, Novartis, Biogen, Amgen, ... -
Hot Plasmids: Summer 2025
TypeBlog PostPublishedAug. 28, 2025, 1:15 p.m....AAV6 is reported to have broad tissue tropism, including skeletal and cardiac muscle, airway epithelial... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m.... 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector, type III with a Cas10... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....effectors allows for a wide variety of screens, including interference, activation, and knockout. Most... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....be produced in multiple different cell types, including plants, mammals, and insects. In this post, we... -
Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....more. The team shares results early and often, including works-in-progress still in need of further study... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in ... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....fluorescent output. The GEVI family of gadgets includes Ace, JEDI, and many more. They can be targeted... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....virtual and hybrid options is more accessible, inclusive, and accommodating for everyone involved in the... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....qPCR. qPCR is used in a variety of applications including gene expression profiling, studying copy number... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....multiple avenues for professional development including workshops and networking events. I took advantage... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....additional resources provided by the depositor including genbank files and protocols here. Articles Citing... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....available in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid...