We narrowed to 751 results for: Nes;
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TypeBlog PostPublishedApril 16, 2024, 1:15 p.m....amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine amino acid...the fluorophore’s microenvironment to enhance brightness, stability, or other properties is an active ...
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Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....Statistics – The basics Statistical analysis is what determines if there is statistical significance among data...There are many, many statistical tests beyond the ones detailed in this article. These are just some of... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m..... Yu, J., et al., Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007....duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced ... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....of prime editing tools. We’ll summarize the major ones in Addgene’s repository later in the post. Advantages...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m.... knowledge to inform on the qualities of what determines good sgRNA design. Incidentally, the first software...Cas-OFFinder will suffice. Cas-OFFinder provides a bare-bones data dump of ALL strings in a genome using any IUPAC... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....above 30% for a variety of targets in human cell lines, with an average indel frequency of only 1.1%. These...which is significantly more effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog PostPublishedApril 8, 2021, 7:25 p.m....grade DNA! This is just the first of many milestones Addgene is poised to reach in the near future... -
2022 Depositor Week
TypeBlog PostPublishedDec. 12, 2022, 2:15 p.m....this is it!) Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame Thursday... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog PostPublishedSept. 8, 2017, 7:52 p.m....Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on ... -
It’s CRISPR Week at Addgene!
TypeBlog PostPublishedMay 13, 2019, 12:01 p.m....focus on generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog PostPublishedDec. 5, 2023, 2:15 p.m....)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie... -
New FLIS Plasmid (April Fools 2022)
TypeBlog PostPublishedApril 1, 2022, 1:15 p.m....Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that can be activated... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m....) ourselves back into our regular post-holiday routines, why not take a little time to appreciate the ... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m....from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape... -
Enabling high school research at the Journal of Emerging Investigators
TypeBlog PostPublishedSept. 5, 2018, 12:01 p.m....published their work on a protein required for cytokinesis in JEI. Developing an interested in biology, ... -
Expanded Access to Rabies Plasmids
TypeBlog PostPublishedFeb. 1, 2024, 2:15 p.m....will be removed before your account is charged. Timelines We have offered VSV and SARS-COV-1 plasmids under... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog PostPublishedAug. 17, 2021, 1:15 p.m....emotions (while you’re on a break from expressing some genes of interest). Blugene is so excited they... -
Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart
TypeBlog PostPublishedMarch 9, 2018, 2 p.m....creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...