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We narrowed to 790 results for: Por

Showing: 561 - 580 of 790 results

  1. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...about publications in the fields of research they support. Monitoring these types of accounts can help you...and figures using machine learning to extract important features such as influential citations, images...

  2. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...of AAAS, WCSJ or ECSJ. These meeting are THE opportunity to grow your network. Get to know and get known...or newspaper (here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship, which places...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...Perturbation Platform, to produce their shRNA library to support functional investigations of the mammalian genome...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

  5. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...lines can be found in the Cell Catalog on our data portal. The plasmids should be functional in many different...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...grown in high concentrations of IL-3 show a high proportion of blue fluorescence, indicating cell proliferation...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

  10. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

  12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....

  13. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...area (the amount of agarose/DMEM should be proportionately increased). For some cells, add a drop of serum...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...cells that have randomly integrated all or large portions of the plasmid. Deliver your construct to your...marker, but cells with random recombination may incorporate the negative selection marker into the genome...

  17. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...

  19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... accidental release. Science relies on popular support, which in turn depends on widespread trust in scientists...environment, they must never be used without popular support2. An accidental release could delay real-world ...

  20. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...have that conversation out in the open is really important. Addgene: Is there anything else you’d like to...biology is massively underfunded considering how important it is. I would really like to reach all those ...
Showing: 561 - 580 of 790 results