We narrowed to 855 results for: REN
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TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....assistance with this blog post. References and resources References Feng, J., Zhang, C., Lischinsky, ... nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly permuted...
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Supporting Rare Disease Research with BeHeard 2017
TypeBlog PostPublishedJuly 20, 2017, 2:30 p.m....in diabetes and lymphoma research or the many different ways you can improve your heart health. These .... Like other forms of mesothelioma, there are currently no targeted treatments for peritoneal mesothelioma...rare disease research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352: Systematic... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m.... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m.... more than 75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years...over the first five months of 2014 reaffirms this trend. In addition to this, more and more European scientists...focused on supporting European scientists. Born a french frog, I moved to Albion six years ago to work as... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend less...plasmid use in the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, ...a plasmid vector, molecular biology tools and references, and how to maintain your plasmid stocks. The... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m.... the biomedical research field! References and Resources References Fellmann, C., et al., Cornerstones...knockout in a cancerous background and treat the parental cell line and the X knockout with your drug(s)... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog PostPublishedNov. 1, 2016, 2:30 p.m....guest blogger, Meghan Monroy. Meghan Monroy is currently an Outreach Data Curation Intern at Addgene. She...Connecticut interested in protein science. References: 1. Celie, Patrick HN, Annabel HA Parret, and...Recombinant cloning strategies for protein expression." Current Opinion in Structural Biology 38 (2016): 145-154... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog PostPublishedOct. 18, 2017, 1 p.m....science writing is clear, Caitlin’s career plans weren’t concrete when she finished grad school at the University...enjoyed the writing and editing sphere.” But things aren’t always that simple, especially when your spouse...growing in the science sphere, but the journals aren’t out here. They’re really East Coast. When we first... -
Management for Scientists: Giving Feedback
TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m....feedback (may be the same as self-assessment or different): Here are the 3 things I would say worked out...you needed? What could I (manager) have done differently? What Can You Learn Going Forward: If you could...could do the thing all over, what would you do differently? Beware of indirect language You must be honest... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....in this series. Check out Joanne's Reddit AMA Different types of mentors for grad school vs. postdoc Choosing...Choosing an advisor to complete a successful PhD is different from choosing a lab for postdoctoral work. However...think, “Oh, but that won’t happen to me – I am different.” Unfortunately, a bad mentor can be bad news... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....each of the gRNAs. The idea was to assign four different barcodes to each of the gRNAs and to embed these...of the recombination sites in the construct to different lengths, the density of cells that are labeled...identify new antifungals that target calcineurin. Current calcineurin gene reporters are based on the bacterial... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....diseases. “Today, I am a scientist that helps with different aspects of manufacturing and quality, especially...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ... and former Associate Director of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....initial system by using a stronger promoter and different biochemical secondary energy sources to increase...XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry is used to sort populations...XMAS-TREE-based methods at various genomic loci in four different categories. Brookhouser, N., et al. BMC... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....will help with the next steps. References and resources References Al-Allaf FA, Tolmachov OE, Zambetti...Choose a restriction enzyme that will give you different band patterns for the recombined vector backbone... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m.... if I would have formulated specific comment differently if I would be speaking to a colleague in the ...of Arabidopsis HapMap population in Amsterdam. Currently, she is a postdoc at KAUST, Saudi Arabia, in the...own research group at Boyce Tompson Institute. References Baldwin, Melinda. "Credibility, peer review, ... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....why this protein was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from... are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and N- termini of...community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (...2013). However, the brightness of Brainbow was inherently low. This is because the stochastic and combinatorial...website: https://dn-en.med.kyushu-u.ac.jp/ References Cai, Dawen, et al. "Improved tools for the Brainbow... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....out if you want to learn more. References and resources References Balloux, F., & van Dorp, L. (2017...and an enveloped virus (right). Created using biorender.com If you’re new to talking about viruses, here... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m.... together as useful and productive, forming a different constellation of interests and skills than what...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...biology. Beyond that, she is also a fan of baseball, French horn, and rhubarb. Follow her on Twitter @Pam_Hines... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....sections and brought in more focus on the list of conferences I presented at and placed them under headlines...before I went for the interview. So reading for my current sales position interview was just as important....mechanisms of cell division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on ...