We narrowed to 602 results for: REV;
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TypeBlog PostPublishedMay 20, 2025, 1:15 p.m.... used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially...
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Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....deeply interested in pursuing them. We’ve recently revamped our viral vector selection process to standardize... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....set up. Then they block the capture reagent to prevent non-specific interactions. Next comes the binding... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m.... applying to. Be clean and professional. Always review grammar and spelling several times and ask someone... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.” Chem Biol... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m....journal article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m.... Labor to update the FLSA, which had not seen revisions since 2004. In July 2015, the Department of Labor... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....circumvent sequencing and the need for a DSB in their previous reporter, the Harris Lab created a panel of eGFP... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog PostPublishedApril 16, 2020, 1 p.m.... RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....Dr. Amanda L. Gunn and Dr. Denise M. Akob for reviewing and commenting. Binning Singletons is especially... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....Holmes, Christina, and William L. Stanford. "Concise review: stem cell antigen‐1: expression, function, and... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....skills are the non-technical skills you pick up wherever you work, and you’ve almost certainly developed... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....groups to bases at specific nucleotide sequences, preventing the cognate restriction enzyme from cleaving ... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....phosphatases that may degrade the target protein. To prevent this, include protease and phosphatase inhibitors... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....rapidly iterate and optimize our pipeline. After reviewing and testing several platforms for engineering ... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....fluorescent protein fusions in live cells in real time. Previously, we have described a number of practical considerations... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....aliquot/preparation of the test plasmid. Let us now revisit our experiment. In Figure 1, it appeared as though... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m.... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....structure, function, and regulation. Biophysical Reviews, 13(6), 1081–1090. https://doi.org/10.1007/s12551...