We narrowed to 837 results for: RING
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TypeBlog PostPublishedSept. 2, 2014, 3:05 p.m....few of these trainees are sufficiently prepared during their 6-12+ (!) years of training to get jobs. ...designing career exploration programs or peer mentoring groups. But for now, you can click here to register...
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6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog PostPublishedJune 26, 2014, 2:23 p.m....published on BitesizeBio here. As part of my job ensuring plasmid quality at Addgene, I analyze 50-100 sequencing... difficult template. Anything more and you’re venturing into the uncertain terrain. 4. Never trust the... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m.... service. We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared...19 pandemic, we saw the need to expand reagent sharing even broader and began working with tech transfer... -
Your Top Requested Plasmid in 2016!
TypeBlog PostPublishedJan. 11, 2017, 8:47 p.m....2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....the detection of RNA, dsDNA, and now ssDNA. By comparing the ability of Cas12 and Cas14 to detect SNPs ...infections, cancer, and other diseases. References 1. Harrington, Lucas B., et al. "Programmed DNA destruction... -
Microbes: Look, Listen, and Tell
TypeBlog PostPublishedAug. 28, 2018, 12:04 p.m....ultrasound. In his research he applies naturally occurring bacterial “molecular buoyancy devices” (also known... Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog PostPublishedJan. 28, 2014, 3:10 p.m....reference: OpenWetWare Preparing chemically competent cells (protocol) Preparing electrocompetent cells... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m..... “What is different is the plasmids you use to bring this into your target organism. We have designed...,” Lohmann said. “We strongly believe the free sharing of material is absolutely essential for scientific... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann...flexible their LITE system could be by applying it to altering gene expression via epigenetics with the addition... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....Farewell 2019, and hello 2020! As we're gearing up to start a new decade, we’re also looking back at ...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....viral service in late 2016 and requests are still pouring in! While our lentiviral service is going strong...which cells are successfully infected by an AAV by altering your injection site and choosing a specific serotype... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....broken down below for specific topics discussed during the interview. Happy listening! Listen to the interview... Sit'N Listen - In their podcast, the SITN team brings in scientists from a variety of fields as well ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson’s and... Mary Gearing... -
Easing the Protein Purification Process with pCri
TypeBlog PostPublishedJune 19, 2015, 3:08 p.m.... the RCSB Protein Data Bank and PDBsum. After acquiring this information, you can then pick an expression... Mary Gearing... -
Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m....www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert G...smaller competitions are now taking place and gathering momentum, including in Cambridge and Bristol. ... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory elements...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania. References... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....the sensitivity of nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with.... (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nature Methods, 20(9)... -
What's New in CRISPR - March 2020
TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m..... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m.... tempting to rely on the citation number when comparing two plasmids, we recommend reviewing the cited...depositors, giving us as much information as possible during the deposit process. Special collections pages ... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m....Eric Lander, comprise 73,171 guide RNAs (gRNAs) covering a total of 7,114 human genes. Unlike the GeCKO...cycle proteins, and more. “In the past, genome engineering with CRISPRs was limited to modification of a...