We narrowed to 852 results for: STI;

...plasmid due to improper plasmid partitioning, it will still inherit some toxin and antitoxin. However, the antitoxin... serves as a counterselectable marker in the destination vector. Successful cloning occurs when the ccdB...to apply this system as an orally administered diagnostic that can indicate the presence of liver metastasis...

...control over when and where the enzyme is active, drastically reducing background labeling caused by the biotin...that had been trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023...roles in synapse development, transmission, and plasticity and is a popular marker of excitatory synapses...

...available to researchers around the world. While we still offer many resources for producing vectors from ...vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023, we shared 93,491...difficult as it may be to find answers to such questions, Addgene is deeply interested in pursuing them...

...numerous fields including research and development, diagnostics, drug discovery, and food safety.  Part of the...antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that they do not ...is non-specific binding or false positives.  If testing a recombinant protein, especially one with tags...

...faster, and more efficient than previous methods. Still, generating reliable results from CRISPR edit data...comparing the robustness, accuracy, and speed with existing tools. For example, we examined ICE analysis of...contact us at [email protected] if you have any questions, and check out the Synthego blog for more great...

...attention, though in reality much of our research still fails to reach our primary target audience – colleagues... disciplines and the years. Which leads to the question of how we can support researchers as they try ...much anyone applying the scientific method to a question. Videos from any discipline are welcome. Up to...

...specific operational or commercial challenges or questions that clients face imminently. Consulting firms...research, drugs, the diseases they can treat, and diagnostic advancements. Consultants can help these teams...weight with your scientific expertise. Do I have to stick with consulting in the long-term? A few years as...

...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...feature genetic, physiological, or developmental characteristics that make them ideal for studying in a laboratory...regenerate, the axolotl has several attractive characteristics that make them an ideal model organism; they...

...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...urotensin. The MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved...better response kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and... to the potassium concentrations in cells than existing sensors. Using genome mining and structure-guided...

...this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...Central PMCID: PMC5568673. 8. Howes, Rob, and Christine Schofield. "Genome engineering using adeno-associated...

...focused scanning libraries. PFunkel: Of prior existing comprehensive mutagenesis methods, PFunkel offers...). If so, the nicking mutagenesis protocol will still work if the orientation of the BbvCI site(s) of .... PubMed PMID: 20676978. 3. Wrenbeck, Emily E., Justin R. Klesmith, James A. Stapleton, Adebola Adeniran...

... unbound by protein. These unbound regions are “sticky” and have the potential to bind nonspecifically...milk, bovine serum albumin or other proteins. The sticky areas on the membrane will bind to the proteins... thoroughly. Vendors will frequently include suggestions for working concentrations, blocking buffers,...

...quantification). But what if you’ve got several interesting protein targets and you want to know if they ...fluorophores, then labeling with the other, you can still record the signal separately for each target.   ...your next multiplex IF experiment! If you have questions or any favorite tips and tricks, leave a comment...

...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable...found that mating improved editing efficiency suggesting that sperm are more permissive to Cas9 editing...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

...tool for predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

... to test the selectivity of your antibody. But testing is not one size fits all. The approaches used will...found that it is often best to determine this by testing them. For intracellular targets, we usually test.... The specific approach will depend on the characteristics of the target and whether suitable cell types...

...-019-0193-0 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...variants to develop five new base editors with distinct PAM sequences, expanding the number of available...

...concepts. Each short video answers an Antibodies 101 question, like "what is an antibody?" or "what is an IgE...scicomm ♬ original sound - Addgene We'll be posting these animations regularly from now until May, ...

...decorating your lab for the upcoming wintertime festivities! Help us spread some cheer by snapping a photo...Christmas, Hanukkah, ugly sweaters, Flannel Fridays, Festivus— you name it, we want to see it.      All photos...