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Addgene
Showing: 561 - 580 of 803 results

  1. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...diseases, offering them the chance to win some of the latest tools and technologies in life sciences research... other genes involved in photosensitivity in melanocytes with the goal of understanding both XP and melanoma...

  2. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...been limited to the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae...regulator or the number of designed scaffold binding sites. With hundreds of characterized aptamer domains ...

  3. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... for marketing positions posted on recruitment websites, this job specifically looked for a researcher...think of to start was “Marketing for Dummies”. The fastest way to learn something about marketing would be...

  5. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...on a web-based interactive visualization of FP attributes (which turned into www.fpvis.org).  That page...is also difficult to scale such a resource when updates depend on a single person. With thousands of scientists...

  6. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...nearby resulting in fluorescence within tens of minutes (Cabantous et al., 2013). The Shu Lab took advantage...reporter can be used to visualize apoptosis of enterocytes in the midgut of Drosophila, an observation that...

  7. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...layer of both bacteria types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively...the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer, trapping the large dye...

  8. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...has been sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary...

  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point...

  10. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...Pomodoro technique, which is when you spend 25 minutes focusing on a task, like writing, and then take...managers, but Mendeley is free to use. You can take notes and highlight the papers saved to your Mendeley ...

  11. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ... all worked in tandem, within their respective mandates, to tackle a controversial and ethically fraught...stages of human clinical trials. This model also demonstrates that when we are at the point of scientifically...

  14. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations...

  15. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where...

  16. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are...

  17. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ...

  18. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes...

  19. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...
Showing: 561 - 580 of 803 results