We narrowed to 815 results for: TES
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TypeBlog PostPublishedNov. 18, 2016, 3 p.m.... Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and ...Medical School describes how AAVs function and indicates important things to consider if you’re getting...
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Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....applied for identification of essential MYC binding sites in a wide range of human cells. A new pooled library...targeting start codons, splice acceptor and donor sites, and introducing premature stop codons. New... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,000 genes in disease models... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein ...through successive rounds of mutagenesis for the brightest teal fluorescent proteins that were photostable... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for...the cytosol, this ratio is low; but when Keima accumulates in autolysosomes, this ratio is high. When untargeted... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m....faster with technology transfer offices (TTOs). The latest of these efforts is the Golden Stopwatch Award ...Montana State University National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) National... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....KAUST, Saudi Arabia, in the group of Prof. Mark Tester, where she focuses on (1) salt-induced changes ...Credibility, peer review, and Nature, 1945–1990." Notes and Records: The Royal Society Journal of the History... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog PostPublishedNov. 8, 2018, 3:47 p.m.... was natural or synthetic 97.7% of the time in a test set of 173 known synthetic and natural genes. ...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m.... described by Li ZY, et al. PLoS One, 2016) and tested a series of mutations. Through this process mCarmine... mNeptune684, and has a favorable pKa of 5.6. To test out this new FP in your own experiments, mCarmine... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....again until you don’t.” As every researcher can attest to, science is nothing if not solving your way ...creativity, changed the outcome of the project, or illustrates the value of the work and then build a story ... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....diseases, offering them the chance to win some of the latest tools and technologies in life sciences research... other genes involved in photosensitivity in melanocytes with the goal of understanding both XP and melanoma... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....been limited to the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae...regulator or the number of designed scaffold binding sites. With hundreds of characterized aptamer domains ... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m.... for marketing positions posted on recruitment websites, this job specifically looked for a researcher...think of to start was “Marketing for Dummies”. The fastest way to learn something about marketing would be... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions that motivate...neuron or set of neurons, you can see how it contributes to behavior or pathology, and by disabling neurons... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...has been sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....on a web-based interactive visualization of FP attributes (which turned into www.fpvis.org). That page...is also difficult to scale such a resource when updates depend on a single person. With thousands of scientists... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....under a new promoter each time a new hypothesis is tested. Controlling multiple genes with Gal4/UAS system...Targeted gene expression as a means of altering cell fates and generating dominant phenotypes., Development... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m.... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are...