Skip to main content
Addgene
Showing: 561 - 580 of 797 results

  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially stained with two HRP...expensive Can only stain with one HRP-RANbody at a time GFP, RFP, H2A2B, Gelsolin Spaghetti Monster: ...

  2. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...advantage of this alternative is the possibility of real-time imaging. Robert Singer’s lab at Albert Einstein ...tag after pulse labeling of a determined period of time. Thus, only newly synthesized proteins of interest...

  3. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...the activity or function of a single protein? Oftentimes, they rely on protein purification. In this strategy...to the metal ions once the protein is folded. Sometimes a tag placed on one end will be blocked during...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...have been harnessing the power of dsRNA for a long time, even before we knew about all the diverse in vitro...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...has thus benefitted from countless rounds of optimization. Dozens of variants exist with unique cutting...and diversify PAM sequences of Cas12a to further optimize it for therapeutic and laboratory usage. Fast...

  6. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...usually 1-3 of the first 3 bps are modified) for optimal stability and efficacy.  Base modifications What...mechanism to turn cutting off or on at a specific time. Recent advances in gRNA modification have overcome...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...isolating total RNA, this method requires a lot of time and depending on how many samples you may have, ...isopropanol precipitated. This method reduced the time it took to isolate RNA from 20+ hours to around ...

  8. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...“Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology by integrating... one thing, cells and cell lines degenerate over time—and sex can be hard to determine. To remedy this...

  9. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

  10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ... workhorse protein even more useful! From FPs optimized for oxidizing environments to photoconvertible...sophisticated experimental manipulations, saving the time and money needed to develop new lines. Using nanobodies...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. Selection...expressed only in specified cells or at specified times. Combining this with some of the loxP methods described...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...be required by one or more NRs for efficient and timely activation or repression of gene expression (see...the research literature and acquiring them in a timely manner. Addgene represents an excellent solution...

  13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...for targeting thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA... the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective in reducing...

  14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... a catalytically inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus...recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use lentiviral...

  15. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...interview is complete. Remember, your interviewers took time out of their day to talk with you, so be engaged...

  16. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...not only highlighted new tools but also included time for questions and a group discussion on the best... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

  17. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...modifying these tools for COVID-19 detection with the ultimate goal to develop low cost and quick point-of-care...SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection or learn more about it ...

  18. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...for a year, I’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a...courses in social, health or policy-related topics, as time permits. Also seek out opportunities to communicate...

  19. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...interaction is typically irreversible and requires some time for chromophore maturation, splitFAST displays rapid...Remember that the FP fragments themselves can sometimes interact in cells and produce background signal...
Showing: 561 - 580 of 797 results