We narrowed to 868 results for: TOR

... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...Addgene’s plasmid sharing services, as well as #happydepositors who stopped by to say hello. Needless to say...amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...sphaeroides!) at the ASM booth. As well as being the Director of the Great Lakes Bioenergy Research Center and...

...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...successful cloning. In these instances, the cloning vector typically has two separate resistance cassettes...powder form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated freeze/...not add them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive...

...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants ... more than 100,000 samples to researchers and educators in 60 countries each year. Introducing Arabidopsis...Science and Industry (COSI) and the Franklin Park Conservatory. Do you use Arabidopsis in your research? Are...

...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...the extracellular domain of the erythropoietin receptor and subsequently evaluated interactions with mutant...antibodies against MBP are commercially available. Factor Xa loves calcium, but metal-chelate matrices, not...Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot...

... first step. Developing mentoring relationships, especially with peer mentors, to discuss your strengths...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

...the table below for more details.   Common Vectors Copy Number+ ori Incompatibility Group Control ...Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility groups... ori's are referred to as phagemids.   Other factors that affect plasmid copy number  Although the...affect the copy number of a plasmid, other external factors contribute as well. These considerations are especially...

...). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries is...data prepared. All of these questions relate to factors that you should have already recorded as good reproducible...Chemogenetics) or light (Optogenetics) to activate receptors? If you’re in the process of designing your experiment...Addgene.org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...included a voltmeter on top of the battery box to monitor charge status and a disconnect switch between the... the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down.... can enable applications where reagents must be stored at elevated temperatures for long time periods ...

... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions.... For this reason I generally recommend that laboratories that have already attempted several CRISPR experiments...above or, even better, first-hand knowledge from laboratories employing CRISPR on the same organism could ...CRISPRseek offers great utility and coupled with Bioconductor should be the first choice for anyone already...

...none are lost. Each Addgene library has its own depositor transformation and amplification protocol, but... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...screening, and if you have any tips, input, or stories of interest about library use, let us know in the...

...University, and did postdoctoral work at Harvard Medical School and the University of Toronto. He is an author...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...

... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml This method is quick and simple and ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence...protocols and tips Find the best way to elute and store your plasmid DNA Learn about the different grades...

...Tools for checking grammar and jargon Hemingway Editor  This is a free web-based tool for detecting the... saved to your Mendeley library. The library is stored in the cloud, so you can access it from anywhere...you organize and cite papers. With the Zotero Connector, papers can be saved from Firefox or Chrome browsers...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...

...imaged together, you will need to consider the same factors as when choosing an individual fluorescent protein...previous blog post for more discussion of these factors). In addition, you will also need to choose fluorescent...infrared fluorescent proteins require biliverdin as a cofactor and so you may need to supplement your cells with...received his PhD in biophysics from UCSF in the laboratory of Ronald Vale, after which he was a fellow at...

...light activation, you can’t use fluorescence to monitor processes directly affected by light. Long-term...To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...

... a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5. Nucleic...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you’... out of frame! Scott Findlay is currently a Postdoctoral Fellow at the University of Alberta. He is interested...

...creating a time-stamped research plan in a public repository before conducting the experiment (note: registrations...participating journals before a study is conducted. Editors and reviewers recommend changes, and if everyone...position of having robust, null findings that are notoriously difficult to publish.  With Registered Reports...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...comparison to the 62-90% error rate seen with Cas9 editors. The lab believes that this system could potentially...Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et al, ...live cells remains a challenge due to multiple factors, including limitations of number of flourescent...

...shared the paper with my lab since one of the AAV vectors in the paper seemed like it could help one of my...conference, whether you are attending in person or monitoring that conference via social media. The podcast...BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet would not only...you advance your research or career? Share your story in the comments below!   Additional resources ...

...nanobody. As a proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies... Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this OptoNB-...purified. More OptoNBs that bind cell signaling receptors would offer more levers to pull to control signaling... example, if the OptoNB could bind and block a receptor or a ligand’s activity, then a flip of a light...