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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 718 results for: cel

Showing: 561 - 580 of 718 results

  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...

  5. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    .... This tag is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence...fluorescent marker. The GFP tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence...

  7. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...

  8. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

  9. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...engineering project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge... in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science: Interview with...

  10. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

  11. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...as an opportunity to practice communicating.  Excellent scientists learn from advisers and other mentors...help graduate students in all STEM fields become excellent science communicators. To empower graduate students...

  12. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...

  13. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...text for the example image. We’ll wait!  Done? Excellent! We asked our workshop participants to do the ...heard of alt text before, they all came up with excellent alt text for their practice image. We know; we...

  14. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...

  16. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody...

  17. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...

  19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...research (either because of expertise, equipment, or excellence of the institution). Likewise, if you’re staying...Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial ...
Showing: 561 - 580 of 718 results