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Addgene
Showing: 561 - 580 of 651 results
  1. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura...efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within an interior cavity...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...
  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...
  3. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...
  4. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...genre fiction novella as it does to read an 11-page Cell paper - and sometimes, it might actually take me...
  5. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...This can be pretty complicated given that a single cell has tens of thousands of proteins functioning in...
  6. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated by the ...to receive the Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also ...
  7. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016...
  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what...better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed with caution...
  9. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...our set up. The cooling block is used to keep our cells from warming up too much and dying.  Also, we added...
  10. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Precise and efficient scarless genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI...
Showing: 561 - 580 of 651 results