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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 811 results for: cha

Showing: 561 - 580 of 811 results

  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...the current members to share short-term goals and chat about awesome science. Important factors in the ...is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging, they typically offer...

  3. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... in your professional circle to vouch for the character you painted in your personal statement. Make certain...hand to get you into that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These...

  5. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...

  6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...and David Root from the Broad Institute and Alex Chavez from the Wyss Institute were instrumental in helping...research and discovery, while also pushing us to challenge assumptions, holding us to nonprofit best practices...

  8. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...manipulations (CRISPR, etc.), and finish with the characterization of a function or phenotype in the surviving...significant survival advantage, such as a non-essential mechanism or any pathway with cellular redundancies, a screen...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region...fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications...

  11. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...

  12. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...neurons reside.   Impact for scientists While the mechanisms by which retrograde AAV work are not yet understood...

  14. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...original publication but its name was subsequently changed to clarify that it does not contain the trademarked... information, see the manual provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This...

  15. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...molecular biology and genetics. It can be used to characterize the function of certain regions in a promoter...’ve conducted SDM using some of these methods, chances are you’d like to find a better one. Trehan et ...

  16. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    .... Created with BioRender.com. In later characterization studies, the edited embryos proceeded to the...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

  17. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...MTA system. We also started our blog and YouTube channel in 2013, which continue to grow today. “I generally...Neuroscience 2023 conference. In 2020, the world changed in response to the COVID-19 pandemic, and Addgene...

  18. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking to modify...it perfect for targeting mRNA and useful as a mechanism of transient knockdown of gene expression. This...

  19. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...

  20. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...common method. These proteins bind the IgG heavy chain. Running the serum over a column of resin crosslinked...low abundance or whose conformational state may change. If your protein might become slightly denatured...
Showing: 561 - 580 of 811 results