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We narrowed to 818 results for: cha

Showing: 561 - 580 of 818 results
  1. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn.... If you really got along well, suggest a coffee chat in the near future. Even just one more meeting (...
  2. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...spatially limited studies and can fluctuate with changing culture conditions. The use of low intensity light...References Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl...
  3. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...with progress reports, meeting minutes and plan changes. Written documentation helps make sure everyone...
  4. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...the current members to share short-term goals and chat about awesome science. Important factors in the ...is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging, they typically offer...
  5. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...original publication but its name was subsequently changed to clarify that it does not contain the trademarked... information, see the manual provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This...
  6. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...
  7. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...
  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...and David Root from the Broad Institute and Alex Chavez from the Wyss Institute were instrumental in helping...research and discovery, while also pushing us to challenge assumptions, holding us to nonprofit best practices...
  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...
  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...neurons reside.   Impact for scientists While the mechanisms by which retrograde AAV work are not yet understood...
  11. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...molecular biology and genetics. It can be used to characterize the function of certain regions in a promoter...’ve conducted SDM using some of these methods, chances are you’d like to find a better one. Trehan et ...
  12. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...manipulations (CRISPR, etc.), and finish with the characterization of a function or phenotype in the surviving...significant survival advantage, such as a non-essential mechanism or any pathway with cellular redundancies, a screen...
  13. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...
  14. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region...fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications...
  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... in your professional circle to vouch for the character you painted in your personal statement. Make certain...hand to get you into that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These...
  16. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...MTA system. We also started our blog and YouTube channel in 2013, which continue to grow today. “I generally...Neuroscience 2023 conference. In 2020, the world changed in response to the COVID-19 pandemic, and Addgene...
  17. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...fluorescent measurements from red, blue, and green channels overlaid on a bright-field image. Image from Ma...intrachromosomal loci, a major benchmark in characterizing the 4D nucleome. CRISPRainbow: Scaling up to...
Showing: 561 - 580 of 818 results