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We narrowed to 811 results for: lars

Showing: 561 - 580 of 811 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...vectors are replication-deficient. Thus, a very large number of phagemids would be needed for an in vivo...Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial Population...Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene Editing Using CRISPR...

  2. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ... being used. After learning of it's continued popularity, I decided this protocol would be a worthy first...Addgene has also cataloged a variety of useful molecular cloning protocols, including making LB agar plates...needs. At Addgene, we’ll continue to create new molecular biology educational resources, such as protocols...

  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...are not always positioned correctly to target a particular gene. Furthermore, a target sequence may have... desired target locus. The PAM sequence is of particular concern when trying to edit a gene using homology...cleavage events for the VQR and VRER variants is similar to wild-type SpCas9, indicating that the variants...

  4. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...known to have replicons with broad host ranges. Popular replicons from these groups include: RK2 (IncP)...vectors for gene expression in bacteria. Methods in Molecular Biology (Clifton, N.J.), 765, 327–343. https:/...endonucleases on the broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in... variable and helps ensure the phenotype isn’t singular or associated with only one aliquot/preparation...

  6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis Protocols, pg. 103...Measuring the Activity of Protein Variants on a Large Scale Using Deep Mutational Scanning.” Nature Protocols...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ... matter how many times I say this, candidates regularly try to convince me that their search committee...whiskies, but I remember the days of modest postdoc salaries all too well. If there's a group to make money...search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

  8. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ..., but I always seek out information about the particular audience and venue before tailoring each presentation...peer mentors to check your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities...informal poll by asking for a show of hands on a particular question. I like to ask about how many people...

  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use...

  10. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but with some extra...selecting an antibody, some of the requirements are similar to standard IF. Here, we’ll walk you through the...if the localization and intensity patterns are similar between the two experiments. If not, it might be...

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

  12. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...Mentorship Program work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant...President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as... Medical School, where she studies age-related macular degeneration. She is also a blogger who writes ...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

  15. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...been an important animal model for research, particularly genetics research, for decades. Its short life...bright burst of light, visible to the naked eye, at regular intervals. This innovation system can help fly ...

  19. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene... (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038...

  20. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...two blog posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits ...
Showing: 561 - 580 of 811 results