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  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector, type III with a Cas10...

  4. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...effectors allows for a wide variety of screens, including interference, activation, and knockout. Most...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in ...

  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...fluorescent output. The GEVI family of gadgets includes Ace, JEDI, and many more. They can be targeted...

  9. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...virtual and hybrid options is more accessible, inclusive, and accommodating for everyone involved in the...

  11. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...additional resources provided by the depositor including genbank files and protocols here. Articles Citing...

  12. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...available in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid...

  14. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use...

  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 ...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...identification of several disease-causing genes, including the RPE65 gene responsible for an autosomal recessive...

  18. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...mammalian cells. The modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation...

  19. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...transformation method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium...
Showing: 561 - 580 of 619 results