We narrowed to 783 results for: INA

...specificity in retinal cells. To do this, the group developed a new strategy for finding retinal cell-type ...monkey, and human retinas Next, the 113 AAV promoters that were active in mouse retinas were injected into...human retinas but not in mice was low (R=0.12), suggesting that mice could be used to eliminate promoters...gene expression in retinal cell types and classes in mice, primate, and human retinas. These synthetic ...types, such as inhibitory neurons, rods, cones, or retinal ganglion. Rather than using naturally occurring...they drove cell type specific expression in the retina.  Four approaches for designing synthetic promoters... with sequences upstream of the start codon of retinal cell-type specific genes from mice. Strategy #2...

...expression of other recombinases. Multiple orthogonal attB-attP pairs per recombinase may be arranged on...different recombinases are overlapping or nested on a register, then the operation of one recombinase on the... when treated with different combinations and orders of two recombinases. How might one read the state...they can control recombinase activity. For example, post-transcriptional recombinase activation via RNA...reduces the content of our paper, “Synthetic recombinase-based state machines in living cells” (1), into...explanation of how to adapt our engineered devices, recombinase-based state machines for your own experimental... about the order or timing of these factors. Recombinase-based state machines (RSMs), which take on a ...

...Having never been to China myself, I was pleased to be able to participate when the organizers of the...Dmitry Samarsky, and a very welcoming team from China’s Ribobio, the illustrious speaker list attracted...winners, one NIH Director and a co-founder of BGI-China presenting, it’s no wonder it was attended by over...were potential Addgene requestors and depositors. China is in one of the top 10 countries in number of plasmid...of Guangzhou and see some of the sites. Most fascinating to me was the outdoor market where one can shop...chemistry is complex and this work requires an interdisciplinary team.  Day two of the conference was kicked...from different species for genome engineering. The final keynote presentation was by Nobel Laureate Thomas...

...iGEM team originally used a guaB knockout E. coli strain to assay for functional decaffeination in cells...supplemented with various caffeinated beverages, but not with caffeine-free drinks. Finally, they demonstrated... pulse of blue light, and is reversible upon termination of light stimulation. CRY2clust enables faster... changes in intracellular Ca2+ levels than the original version containing CRY2 without the extra peptide..., termied soCoChR, by fusing a fragment of the kainate receptor subunit to the C-terminus of the protein...the group was able to demonstrate that while illumination of neighboring cells evokes action potentials...these APs are absent in soCoChR-expressing cells. Finally, using synaptic receptor blockers to distinguish...

...plasmid-based recombinant monoclonal antibodies Resources on Addgene.org Find recombinant antibody plasmids...leading to problems with reproducibility and an inability to improve upon these critical research tools.... Addgene to create a much needed open-access recombinant antibody/affinity reagent resource for the neuroscience...and data, the platform can be expanded for any recombinant antibody tool and therefore will grow to serve... the entire scientific community.     Why recombinant antibodies? Animal-based methods for antibody...cryopreservation (Bradbury & Plückthun, 2015a, b). Recombinant antibodies are plasmid-based and offer numerous...monoclonal antibodies. You can learn more about recombinant antibodies in this blog post. Hello, NABOR! ...

...Fvasconcellos.     How are Recombinant Antibodies Made? To make recombinant antibodies, you first need..., 2015).    The Many Benefits of Recombinant Antibodies Recombinant antibodies offer numerous advantages...definition of recombinant antibodies are essential for good reproducibility. Non-recombinant antibodies ...predict a similar revolution for antibodies. Recombinant antibody technology makes it easy for scientists...components of the scientific toolbox. What are Recombinant Antibodies? Antibodies are used by scientists...scientific institutions have proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun...single antibody recognizing one epitope. (Bottom) Recombinant monoclonal antibodies are encoded in plasmids...

... multiple Addgenies and most recently updated by Ina Ersing in August 2023. Resources and references ...  Ina Ersing...absence of unexpected DNA contaminants. The data is also used to determine recombination rates in FLEX/DIO/fDIO...Endotoxin contamination in vector preparations can alter the immunogenic properties of the final product...mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event...is performed on viral genomes isolated from the final AAV preparation. Sequencing results are analyzed...particularly in large animal studies. Endotoxin contamination is minimized by using an endotoxin-free plasmid...

..., Diana, Dolly, Eddy, Emma, Eric, Gizela, Henry, Ina, Inna, Ishrat, Jane, Jason, Jessica, Joel, Jonathan... revenue to build reserves so the company is sustainable. The difference is what nonprofits do with their..., Lianna, Luke, Marcy, Mary, Max, Maya, Meghan, Melina, Meron, Michael Lo., Michael Le., Michel, Michelle...

...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources...behind Mycobacterium tuberculosis resistance to pyrazinamide (PZA) (a drug commonly used to treat tuberculosis... different approaches, including CRISPR and recombination-mediated genetic engineering (find more information...pathogenic Klebsiella and Pseudomonas isolates causing urinary tract infections. This project aims to examine ...Adeyi at the University of Ibadan. She works with medicinal plants such as Launaea taraxacifolia (Willd), ...pathophysiological complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation...Ernesto Valdivia Silva is a master of interesting combinations: biomedicine and astrobiology. During the last...

...series!  This post was originally written by Didem Goz Ayturk and updated by Ina Ersing in August 2023 ... your final stocks only contain rAAV. To minimize contamination, use appropriate decontamination procedures...40% PEG solution to each 100 mL of supernatant (final concentration of 8%). If, however, you’ve collected...digital PCR (ddPCR) (Lock et al., 2014), or determination of the median tissue culture infective dose ...primers targeting the viral genome or inverted terminal repeats (ITR). You should always have a high-quality...adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy Methods, ...Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular...