...As Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working...proteins. There are still things we haven’t solved that stop it from being used. One thing is that Cas9 is toxic...

...Michael Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer...

... and so the lab organized a sampling trip to Yellowstone National Park (YNP). Extremophiles - phylogenetically... can enable applications where reagents must be stored at elevated temperatures for long time periods ...

...graduate work in Professor Jim Collins' lab (then at Boston University, now at MIT) where he worked on understanding...

...Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including only...contaminating AAV DNA. In addition, we wrote an open-source custom Python script that uses the VGS data to determine...data in minutes and the scripts  can be easily customized to detect other sequences of interest.   While...

...lung, the heart, and the abdomen. Long story short, when asbestos is breathed in (or less frequently, ingested...small, hook-like shape of asbestos fibers. Over time these embedded asbestos fibers cause inflammation...known safe level of exposure to asbestos – i.e., any exposure to asbestos is potentially capable of causing...exposed to asbestos but have not developed the cancer yet Those who have not been exposed to asbestos Tests...greatly decreased through a ban on asbestos. Nonprofits like the Asbestos Disease Awareness Organization ...bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that future generations...completely preventable. Mainly caused by exposure to asbestos, mesothelioma is a form of cancer that develops...

...Having spent that long in customer service, I've daydreamed about the ideal customer service interaction -...to any biology-related customer support interaction. Before contacting customer service In an age of Tweeting...answered hundreds of phone calls and thousands of customer service emails in my six years as a senior scientist... - one that gives our customers the most utility in the least amount of time. Though I now spend far less...my years of accumulated wisdom so that you, the customer, can get the most out of your email or phone call... bad product (in which case, you should probably stop using that company). Frequently asked question pages...section are really, truly frequently asked by customers (and I expect this is the case at most companies...

...pH. We compared the dry ice-stored samples to controls that were only stored in a -80°C freezer and not...of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results showed that...mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and...reading a publication describing how short term storage on dry ice can shift sample pH, we wondered whether...freezer and then moved them to a closed dry ice storage container for 48 hours (to replicate how our viruses...from the dry ice). After 48 hours of CO2/dry ice storage, we then thawed the samples at room temperature...thawed, there was no effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once...

...then inoculated for storage on Thursday. The lab prepares bacterial glycerol stocks on Fridays. From each...used for long term storage (one is kept at Addgene and another is sent to a storage facility). The third...courier and then the journey of transformation and storage begins. Some samples are submitted as bacterial...intact. Some deposits are sent from labs nearby in Boston and Cambridge. Many deposits arrive from labs around...sample can be tracked easily at each step in the storage process. After the original sample is barcoded,...entry into Addgene’s repository. For long-term storage in our facilities, every sample is transferred ... - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control processes...

...support through the customer’s perspective is good. If you’ve never participated in a customer support conversation...the types of questions customers ask, and there are also differences in customers’ personalities and their...reviewing customer data sets, troubleshooting protocols, analyzing information, and solving customer problems... product issue in the lab. The issue was with a custom-made reagent to manipulate cells (manufactured ...multiple languages - that makes communication with customers from around the world easier. You should also ...expectations. If you are willing to listen to a customer’s concerns and are patient enough to find the real...are the most popular ways to communicate with customers. Online live chat is a convenient alternative ...