We narrowed to 884 results for: ica

...re more interested in selling a product than communicating your ideas with people or discussing science...I do a lot of public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn...own expertise and your resources for science communication opportunities like an AMA. One of the best things...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect... science communication...

...protein purification. For a more thorough introduction to pCri, please see the original publication cited...expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology... Protein purification can be one of the most stressful lab activities. Working with proteins requires...interest can save a lot of time later. Protein purification specialists recommend doing some research, notably...Gomis-Rüth, aims to take the guesswork out of protein purification through simple and efficient multiplexing. pCri...sites should one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a ...4a and -9a. 6xHis tags enable affinity purification using a Ni-NTA resin. MBP improves protein solubility...

... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...chief goals. First, SITN works to bridge the communication gap between scientists and the public and help... SITN helps graduate students develop their communication skills and engage with the public. The organization...Bio-Medley) to showcase SITN’s strength in the biomedical sciences and decided to offer a varied format...students, these opportunities to improve our communication and organizational skills provide great training... science communication...

...to a) figure out the sources of miscommunication and b) find practical solutions.  My PhD prepared me... for this task. Good scientists learn how to communicate their work differently based on their audience... family is going to vary widely. The way you communicate your science at a poster is going to vary depending...two main problems with Addgene’s cross team communication. Although these real world examples did not ... for the container to be used in cross-team communication A list of “AKA” names, or jargon terms, for ...types or purposes of each given container, when applicable Any other relevant information Prescribing an...tracking system. Though you might not have to communicate with a team of software engineers in your lab...

...background, which means that each mouse is genetically identical and all mice of the strain are homozygous...create a limited but genetically diverse gene pool. Outbred strains are strategically bred to maintain maximum...using the same nuclease may not result in genetically identical mice. Thus, scientists must genotype each...organism used to understand mammalian traits and genetically engineered mouse models provide researchers with...useful and adaptable tools to perform basic and preclinical research. For scientists new to using mouse models...in research can fall under one of two broad classifications: inbred or outbred. Inbred strains share a ...) National Institutes of Health (NIH) Swiss. Genetically engineered mice Mice can be engineered by inserting...

... Predoctoral Fellow at University of Rochester Medical Center. During development, complex genetic programs...genetic underpinnings of these processes has been complicated by the lack of precise genetic tools for modulating...central nervous system (CNS). To address this technological gap, a trio of recent papers describe the development...recent advancements and offer commentary on their application to neuroscience research. What is CRISPR-based...with different untranslated or coding regions. Typically, each gene isoform has a distinct transcriptional...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly...necessitates such molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids used ...

...some tips for performing this purification.  Check out the AAV Purification protocol page for more detailed...! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation This...This video goes over AAV vector purification using an iodixanol column gradient. We'll show the loading... tissue culture as it is commonly called, is a critical tool for many scientists. Mammalian cells provide...provide scientists with the means to study biological processes on the cellular level instead of having ...

... the Communicating Science Conference series for graduate students. I believe that communication is the...skill in science communication over time.  Like most skills, great science communication comes with practice...able to communicate with their colleagues and peers. Scientists also need to be able to communicate to expert...careers in science communication The strength of story telling Science communication: 9 strategies to get... world is the value that you can succeed in communicating. Even the most rigorous, insightful, and novel...scientist's career is mediated by some kind of communication to another person.  In an interview setting,...you do to ensure that your skills in science communication will be a positive asset throughout your career...

...repair Adapter Ligation Clean up/Size selection Amplification Clean up  I found this kit easy to use because...DNA we started the process with, we got great amplification of our sample libraries. NEB also gave us a ...mostly used to make new libraries as opposed to replicating them like we do at Addgene), going to the workshop...

...and applications.    Plant applications We outlined many synthetic biology MoClo applications, but....” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016, pp. 1059–69. Crossref...Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp. 99–103. Crossref...Libraries.” ACS Synthetic Biology, vol. 6, no. 6, American Chemical Society (ACS), 15 Mar. 2017, pp. 1016–25. ...Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015, pp. 975–86. Crossref...” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, pp. 3393–405. ....” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, pp. 839–43. Crossref...