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We narrowed to 435 results for: Trac

Showing: 61 - 90 of 435 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...block and 62°C heat block Approach: Viral RNA is extracted and subject to isothermal amplification by primers...directly using saliva without having to perform RNA extraction but that may work only if there is a high enough...water bath and 42°C water bath Approach: RNA is extracted from a sample and subject to isothermal recombinase...detect SARS-CoV-2 in the samples. First, RNA is extracted and subject to reverse transcription. This is ...

  2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...has become very useful in lineage tracing; “Zebrabow” was used to trace the development of the corneal epithelium... also been applied to cell marking and lineage tracing in other systems, such as zebrafish and Drosophila...Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish. Pan YA, Freundlich...dev.094631. PubMed. Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R,...

  3. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...companies which are reliant on contracts with clients is that, when those contracts fall through or are cancelled...(SOP’s) and every item I tested was constantly tracked with barcodes and spreadsheets. It was great to...primary purpose of the company was to fulfill contracts, my work sometimes felt rushed and the focus always...company; new staff hired in anticipation of the contract suddenly have less work to do than was anticipated...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...feature of AAVs as a new tool for neuroanatomical tracing (11). With the ability to produce neuron specific...showing a very specific phenotype. Previous neural tracing methods used viruses that were potentially toxic...available, AAVs could be much better tools for neural tracing in very specific cell populations. Find Ready-to-Use...finger nucleases, and many others (12). The most attractive potential of AAVs is their ability to be used...et al. "A fourth generation of neuroanatomical tracing techniques: exploiting the offspring of genetic...

  5. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ... cells is minimal (picogram range). It can be extracted from small bacterial cultures (i.e. 2-3 ml) with...cultures and commercial kits you will adopt for the extraction. Restriction enzyme based approaches can require...and guanidine hydrochloride used during plasmid extraction. Figure 2: Use cloning grade DNA for direct... technique must be larger scale, to allow the extraction of enough plasmid to last for multiple experiments...

  6. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...complement our RNA Extraction Without A Kit blog post, we've compiled five methods for extracting DNA without...that cause plasmids to be incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our...it, wrote these tips for working with RNA and extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection...

  7. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...cells. They bind to proteins on the surface of extracellular pathogens such as parasites or microbes, or ...from the animal is drawn and the antibodies are extracted. These antibodies recognize a variety of epitopes...Hybridomas are formed by fusing B cells that have been extracted from animals injected with protein antigen to ...presence of protein within a sample, you’ll want to extract your protein of interest from the mixture. Antibodies...permeabilized to allow antibodies to bind to intracellular proteins. The fluorescence signal is detected...

  8. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ... deadline again. Organize you experiments, keep track of upcoming grant deadlines, and sync everything...Though electronic lab notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have...allow you to control who can edit the documents and track the revision history. Now you’ll be able to make... knowing the information is backed up and edit tracking will help you identify when others have added ...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...the intensity of fluorescence signal and enables tracking of single molecules within living cells without...thereby creating a single-molecule reporter of intracellular processes. Multimerization via the SunTag system....3147. Bioluminescent pH sensor Understanding intracellular pH regulation is important as pH regulation ...to the cell cycle. Good tools for measuring intracellular pH are required in order to determine the relationships.../Cas9 in genome-engineering. One particularly attractive application involves using dCas9-activator fusion... compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths...synchronous release of the reporters which can then be tracked along the secretory pathway (Figure B). The RUSH...

  10. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...bound to the intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor platform...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ... calculate the amount of bound ligand (after subtracting out non-specific binding). Historically, glutathione-S-transferase...subsequently purify the fusion protein from a bacterial extract. The MBP tag can be removed using Xa protease. ...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...biotin (50X) because 5 mM is barely soluble at RT. Track expression and purification of biotinylated protein...

  12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...barcodes within the gRNA sequences. This allowed tracing of the performance of each gRNA multiple times ...protein phosphatase that responds to rising intracellular calcium levels within the cell. When levels ...required for virulence in pathogenic fungi, it is an attractive drug target. Calcineurin reporters allow scientists... wild type (top panels) and cells with high intracellular Ca2+ (bottom panels) equipped with destabilized...

  13. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ...read on!  What is the MTA?  An MTA is simply a contract or agreement that states the terms of use for ...meaningful progress depends on things being organized, tracked, and clearly communicated. And that’s where MTAs...

  14. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...Simultaneous fluorescence (upper trace) and electrophysiological recordings (lower trace) using OCaMP expressed ...expressed in mouse cortex. Bottom: Zoomed-in view of traces corresponding to the dashed box above. The number ...potential (AP) spiking events are indicated below the trace; asterisk indicates a single AP. B) Averaged fluorescence...

  15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...autophagosomal membranes, so tagging it with GFP allows for tracking the localization or accumulation of pre-autophagosomal...an autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about...easier to study the mechanisms behind autophagy by tracking what’s being transported to the yeast vacuole ...vacuole (i.e. cytosol, mitochondria), rather than tracking generic autophagy markers. It’s particularly useful...

  16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... the crRNA or the tracrRNA. Of course, in a single guide system (crRNA and tracrRNA as a single molecule...region associates with the trans-activating crRNA (tracrRNA) which interacts with the Cas9 protein. These ...you to visualize transfection efficiency and even track localization in cellular experiments. There are ...

  17. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...concentrated by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin...number of 10 cm2 dishes. While this option is attractive because the same procedure for a single 10 cm2...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

  19. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...behind from a preceding animal (ex: urine) can be distracting. If it is not cleaned, animals tested towards...experiment series would behave very differently (distracted) from the ones tested at the beginning. Variability...Both AAV and lentiviral vectors are used for intracerebral injections. AAV particles are smaller and may...liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue damage; however, the...

  20. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...better access to intracellular targets. If your target is already on the extracellular side of the membrane...is suitable for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with...antibodies into the cell where they could bind to intracellular epitopes. C) Permeabilizing with a non-ionic...

  21. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...and requires only one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. In certain...target. Cpf1 also does not require an additional tracrRNA. Once they had determined the minimal elements...favored by SpCas9. Since Cpf1 doesn’t require a tracrRNA, crRNA guides are only ~42 nt long. Direct synthesis...significantly cheaper than that of the ~100 nt crRNA/tracrRNA hybrid guides needed for Cas9 function. Since ...

  22. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...C2c3 are related to Cpf1, but they require both a tracrRNA and crRNA to cleave target DNA (Cpf1 requires ...PAM (PFS) Cleavage Mechanism Cas9 HNH, RuvC TracrRNA, crRNA DNA 5' NGG Specific blunt ended DSB...endogenous CRISPR/Cas9 system, Cas9 uses both a tracrRNA and crRNA to facilitate binding and cleavage of...have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating expression...

  23. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...not recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG...T7RNAP-tetA General protein expression  BLR (DE3) Tetracycline RecA-deficient; best for plasmids with repetative...proteins Origami2 (DE3)** Streptomycin and Tetracycline Contains highly active thioredoxin reductase...troubleshooting measures that can help get you back on track: Compatibility: Double-check your plasmid backbone...

  24. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...that bring more to the table in terms of data traceability and reproducibility of research. Electronic ...work. It is already becoming impossible to keep track of it all in paper notebooks. Following these findings...one place, easily retrievable, searchable and traceable. However, switching from paper to an electronic... place and therefore make it easier for them to track and find data and information. If an employee leaves...

  25. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...fluorescence unit fused with TRansmembrane region-to-IntrAcellular loop linkers” (MTRIA for short), where a fluorescent...inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled receptors....fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain. Nat Methods....  Find B-gTEMP plasmids here!   Lu K, et al. Intracellular Heat Transfer and Thermal Property Revealed ...

  26. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...1885. E. coli are mainly found in the intestinal tract of animals. There are many different naturally occurring... streptomycin Tn10   Confers resistance to tetracycline Additionally, Table 2 provides a quick reference...galE15 galK16 rpsL(StrR) endA1 λ- XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic...lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High competency cloning ...

  27. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...your gene of interest. This can cause issues in tracing studies, where it could potentially label cells...2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator Correlates with Long-Term...vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy, 28(...dual presynaptic inputs using cFork anterograde tracing system. Experimental Neurobiology, 29(3), 219–229...

  28. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...luminescence. Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular Ca++ release...biosensors utilizing NanoLuc® luciferase Many intracellular sensors of events like calcium release have ...Luciferase. CalFlux VNT responds to changes in intracellular calcium and is useful for direct imaging of ...

  29. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...smaller validation screen using selected gRNAs extracted from the same or another library. These different...in animal model systems. Use Green Listed to extract gRNAs that target your list of selected genes. ... design. With Green Listed you can also easily extract gRNAs for the same gene from several libraries,...have different names, and the software can only extract gRNAs for genes using the name that is used by ...

  30. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ... that we can invest our time into learning and extract the most value? This is only one of many decisions...tools assess repair events by type (NHEJ/HDR) and track indels at a single locus, usually the target site...on downstream results. Examples: “HTML”, “visual track”, “plot”, “tabular”, “interactive”, and “save”. ...on your needs. We will likely begin to see a contraction of available tools with more and more features...
Showing: 61 - 90 of 435 results