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We narrowed to 610 results for: STO;

Showing: 101 - 150 of 610 results

  1. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...yourself needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding...with unlimited time? Another strategy for your brainstorm session is to start with something completely... ideas that I have found helpful in my team brainstorming sessions.  Allow the group time to think about...position or the longest tenure, but don’t let that stop you from speaking up.  If your ideas are not well...

  2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow...for improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila...express the same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent... researchers who developed Tetbow. Conclusion Stochastic multicolor cell labeling systems allow single...

  3. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...situ hybridization histochemistry: use for mRNA detection.” The Journal of Histochemistry and Cytochemistry...interaction has also been adapted for use in immunohistochemistry and antibody labelling. In these experiments...referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC...Cytochemistry : Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding needs...alleles in microbial populations through time, Christopher Marx's lab has engineered and validated a new...one-pot reaction. Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil...Abil et al., 2014 show that you can fuse custom PUF proteins to a translational repressor to down-regulate...Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature communications...

  5. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...papers or are brainstorming new technological innovations. Let’s take a look at three historical techniques...that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing...throughout the world. Let us know your favorite "Historical Lab Technique" in the comments section below!...Benjamin Chain. "The sequence of sequencers: The history of sequencing DNA." Genomics (2015). PubMed PMID...

  6. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...Science History Rap Battle A great example of science story telling following the unfortunate story of Rosalind...and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed...

  7. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...the research community. Customers have taken advantage of the customizability, choosing the cargo, serotype... ship them out in record time.  However, our in-stock preps remain a popular choice thanks to the convenience...) Looking for more viral vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Location, Location, Location! Failure to Plan (for Storage) Is Planning to Fail Patience Is Bitter but Its... the transport issues. 2. Failure to plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course...cycles can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze... liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be... PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport of adeno-associated...

  9. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...documenting your plasmids, storing them, providing quality control and customer support, making plasmids...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...The trust of our depositors to allow us to help store and distribute their plasmids. The partnerships ...

  10. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...the CRISPR types and is thought to be the common ancestor of all other CRISPR systems. Type III systems ...(like me!) of CRISPR types — weird and poorly understood. There are three subtypes, A–C, and they are ...mechanism of type II is, unsurprisingly, well understood, and the first taught when introducing CRISPR...Covarrubias, P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini,...., Hunnewell, P., Alfonse, L. E., Carte, J. M., Keston-Smith, E., Sothiselvam, S., Garrity, A. J., Chong...

  11. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...equilibrium” where the cancer stops growing, and the immune system stops fighting. Such quiescent cancer...study cancer from every possible angle to leave no stone unturned, but cancer has been producing landslide...landslide after landslide, only adding more stones to the field. Fortunately, our bodies have been fighting ... downregulate the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces...

  12. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...shared resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens...shared progress. But numbers tell only part of the story. This year, we made significant strides in making... D., Cooper, J. A., Crosby, K., Harvey, K. J., Houston, D. W., Kumaran, R., Rego, M., Schofield, C., …...024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A....

  13. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...have helped Addgene grow and spread our mission.  Boston Museum of Science, where else? Addgenies and other...honored guests headed to the Museum of Science in Boston, where the entire Blue Wing was set aside for the...refresher on the Central Dogma of Biology, I would stop by later this year when it is displayed!).  The ...Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first). After the speeches,...

  14. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...maintain high standards for both our depositors and requestors.  Requesting Plasmids available for Packaged ...quality control assays we run with all of our in-stock viral preps: ddPCR, purity testing, sterility testing...AAV2 to be 7x1012, the same titers found in our in-stock preps. If a request has technical considerations...considerations that may affect expected titers, a customer support specialist will reach out to you during the request...

  15. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...depositors, used by our requestors, and supported by our repository. You can enjoy these stories, and other 20th...rabies plasmids in our repository to international requestors.    I would be remiss not to mention that 2024...Throughout the year, we’ll be celebrating with stories of innovation and impact made possible by the sharing...

  16. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...applications in plants. This smaller, easily customizable vector functions in multiple species, including...for many applications, the plasmid can also be customized due to its modular structure. Two multiple cloning... regulatory elements to be inserted, further customizing gene expression. Sahoo et al. have tested the...Western blotting. Given its versatility and customizability, pSiM24 can be used not only for plant engineering...

  17. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...surely what is one of many awards that will be bestowed upon discovers of CRISPR/Cas9 genome engineering...panel discussion with Dr. Craig Mello and Dr. Paul Stoffels from J&J. The panel was moderated by a non-scientist...done with this technology to help patients. Dr. Stoffels and others in the audience discussed how J&J is...Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution...

  18. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...is especially exciting, including histone acetylation by p300, histone demethylation by LSD1, cytosine ... subpages, our updated CRISPR Guide and CRISPR History provide a great overview of the development of ...resources: CRISPR Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

  19. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...patient partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ ...watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play with my kids... as efficiently in humans. This is why we cannot stop searching for novel solutions to treat rare diseases...against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many new heroes to the...

  20. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab in the ...supply house.  These large, cheap aliquots could be stored indefinitely at room temperature (thus also saving... powder in 50% nitric acid, but I never let that stop me. I only had to replenish my glass milk supplies... required less agar, less ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  ...

  21. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus... The CellTags revealed that cells with common ancestors tended to have similar reprogramming outcomes....results suggest that iEP reprogramming is not stochastic, but rather there’s a particular cell state that...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris...

  22. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...entrepreneur in the health sciences?  Is this a different story altogether? The unfortunate answer is yes, largely...process. I am the cofounder and CEO of miLab, a Boston-based startup focused on empowering early-stage... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...

  23. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...population (pink histogram) compared to the WT cells (green histogram). The unfilled histograms show cells ....3995 Virk, H. S., Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, ...Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P...

  24. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...great deal more information that one might like to store about FPs than is currently represented at fpvis...a centralized, community-editable resource for storage and dissemination of information about FPs. FPbase.org...Users can “favorite” certain proteins, or create custom protein collections (such as a set of FPs that ...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

  25. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...lab meetings with a focus on discussion and brainstorming Though, we might be forced to work from home...of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira.   Implement new...can be used as a break, but can also be used to brainstorm on data in smaller groups, where for instance...

  26. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...resources at Addgene. His background in history and specifically the history of RNAi left him a bit skeptical...Listen to the interview here! Doench majored in history during his undergraduate work at Hamilton College...just been discovered, the mechanism was barely understood.” Doench’s graduate work helped expand our understanding...

  27. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...our guest bloggers Joachim Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...

  28. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...to package reprogramming factors. An important milestone of reprogramming is the silencing of reprogramming...involves making chimeras by injecting iPSCs into blastocysts and implanting them into female mice. Generating...complementation, involves injecting tetraploid blastocysts with iPSCs and measuring their ability to direct...PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving field of induced pluripotency...., and K. Hochedlinger. "Induced pluripotency: history, mechanisms, and applications." Genes & Development...

  29. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ...fluorophore will bleach. This is, however, not the full story. The photobleaching rate does not depend on excitation...our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...

  30. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry may all use either...In the indirect approach, you can simply keep a stock of conjugated secondary antibodies on hand and use...signaling molecules. Many labs find that a trusted stock of secondary antibodies saves time and money when...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals...

  31. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...breast tumorigenesis: Friend or foe?." Histology and histopathology (2018): 18021-18021. PubMed PMID: 29978449...enzymes and metabolism reprogramming aren’t the whole story. It’s essential for a tumor to spread and to resist...sensitive ones. Though this process is not fully understood, a team of researchers lead by Takayuki Kogure...

  32. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo Colombi...probably time to prepare new plates with a fresh stock of antibiotic. Many small colonies surrounding big...University. Oskar Laur, PhD runs Emory University DNA Custom Cloning Service since 2009. He has  developed a...he has used for cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds...

  33. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...Hegazy, et al., 2020; Niemeyer, et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for ...–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous...resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies 101: The Basics of Western...

  34. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...presence of Cre, the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is...with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest...is from Tyler Jack's lab: Lox-Stop-Lox TOPO.  Cre-dependent gene knockout – conversely, putting the loxP...

  35. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...efficient energy maintenance - through acquisition, storage, and utilization - and on self-propagation by reproduction...diabetes, cardiovascular disease, cholesterol gallstone disease, senescent illnesses, and the metabolic...other members of the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. ...sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors, and making them available...

  36. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...blogger Clare O'Connor an Associate Professor at Boston College. National reports stress the importance...yeasts are thought to have diverged from a common ancestor ~350 million years ago. Over time, the two genomes... that is part of the biology core curriculum at Boston College. Students in the course work in teams of...biochemistry, molecular cell biology and genetics at Boston College. She is particularly interested in strategies...

  37. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...a nucleus -> DNA compaction around histones -> Examples of histone-modifying enzymes and reader-effector...repository that will help epigenetic researchers custom design their own engineering plasmid tools in about... specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these modules...

  38. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...Stanford develops engineered DNA systems capable of storing data and computing inside living cells. He's also...describing the development of “transcriptors,” transistor-like digital genetic switches that enable cellular...the beginnings of computational logic and data storage. What we don't have are the keyboards and displays...mining. There is an absolutely incredible hidden story here. The parts we are releasing simply plug into...

  39. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...unevenly, introducing distortions in your final images. It is possible to keep distortions to a minimum with...done about that.  Second, expansion introduces distortions. The goal in expansion is for your sample to ... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

  40. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... convey enough information to tell a compelling story in an image or do you need a full exhibit? In a ....  Static. Can be difficult to convey an entire story or context via still images. Some things cannot ...information. Primarily useful for data-focused stories. In-person lectures, presentations, etc… Low...interested in scicomm. Difficult to do in-depth storytelling. Requires regular content production. Internet...

  41. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency (x-axis) which SNPs ...ancestry-based bias.   The correction process does not stop there. Currently, they are working with Broad's ...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...

  42. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable...affecting many of the RFPs developed from natural ancestors), and screened through libraries of new RFP variants... an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be compatible with two-photon...entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes and were carefully...

  43. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

  44. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...technologies such as array tomography, super-resolution STORM imaging, and electron microscopy. Sample preparation...standard proteins tags like GFP. Specifically for STORM, the high intensity signal provided by smFPs can...Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking and detection: fluorescent...

  45. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...infectious titer. The infectious titer of a viral vector stock can vary based on transduction target and can also...particles were full, compared to 50% of wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-...100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center for Nanoscale Systems, Harvard ...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...

  46. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

  47. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ... done at my first six ASM meetings. I told this story over a great dinner at Pittypat’s Porch with old... career scientists, students, and those from historically underrepresented groups. Through such mentoring...Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa ...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through meetings...

  48. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...single protein, shedding the complexity of its ancestors. They classified the system as Class I, Type III-E...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...editing treatment would have to be individually customized to the patient's specific mutations. With a drag...Kaiyi Jiang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, John A. Walker II, Anastasia P....

  49. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...to inject an Agrobacterium culture through the stomata into the abaxial site (lower surface) of the leaf...MoClo system you can easily and quickly create customized vectors. For more information on MoClo for plants...is a PhD candidate at Northeastern University in Boston, MA. He obtained his BSc and MSc in Plant Biotechnology...

  50. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... payoff for each aim. Learn to communicate your story with only the most salient details. Make the most...your research problem. Creating a coherent unified story and model can help make your ideas easier to understand...Practice presenting your chalk talk Perfect your story and delivery. Design simple figures and diagrams...track. Furthermore, practice getting back to your story. Committee questions can derail your talk. You need...
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