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We narrowed to 117 results for: Cre

Showing: 41 - 60 of 117 results
  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ... For example, a researcher can deliver Cre-dependent TVA to Cre-expressing neurons in a transgenic mouse...viral protein EnvA on its surface will only infect Cre-positive, TVA-expressing cells (Osakada et al., 2013...Gene Delivery to the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control...
  2. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct...
  3. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...interneurons can be labelled with an AAV1 Cre virus combined with a Cre-dependent AAV9 tdTomato virus in the...
  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Addgene depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like... Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E.... Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...
  5. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...control when you turn off expression for good, the Cre-Lox system offers inducible yet permanent gene excision...can be excised by intentional introduction of the Cre recombinase. This is a time-tested system but does...some permanent knock out plasmids? Check out our Cre vector and Murine LoxP vector.   Turn it off for ...
  6. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE)...-00969-4.   Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE)...previously developed the multiplexed version of the Cre-mediated AAV Targeted Evolution method (M-CREATE)...
  7. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...doxycycline, but it can also be regulated spatially with Cre-recombination. Read the paper in Frontiers in Molecular...Averof lab. It’s an alternative to the conventional Cre or Flp recombinases used to excise markers. It has...
  8. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...open-reading frame (DIO). Upon coexpression with cre recombinase (either from a mouse line or viral co-injection...type specificity dependent on the promoter of the cre recombinase while still taking advantage of the robust...an appropriate mouse line expressing a particular cre, viral expression of your opsin is no longer required...tracing with fluorescent proteins in nontransgenic and cre driver mice. Current protocols in neuroscience / ...: PMC4242517.  7. Madisen L, et al. A toolbox of Cre-dependent optogenetic transgenic mice for light-induced...
  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...often made Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre in only...labelling the subset of infected cells that also express Cre. Another advantage of AAV vectors is that there are... traced neurons with an AAV9 vector expressing a Cre-dependent fluorescent marker. Injection into a conditional...conditional mutant mouse brain that expressed Cre recombinase only in pyramidal cells of the hippocampal...PMID: 4107258. Lo, L., and Anderson, D.J. (2011). A Cre-dependent, anterograde transsynaptic viral tracer...
Showing: 41 - 60 of 117 results