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We narrowed to 180 results for: antibody

Showing: 41 - 60 of 180 results

  1. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... repeatedly stripped of an antibody and reprobed with different antibodies allowing multiple targets to...binding to the antibodies during the stain. The ideal blocking buffer depends on the antibodies and labels...your primary antibody Once the membrane has been blocked, it is stained with a primary antibody against your...interest. But which antibody should you use? You will likely find several different antibody options targeting... their antibody binds to protein of the correct size. Many vendors will show that the antibody binds to...gold standard in antibody validation. This level of validation ensures that the antibody is staining the...help of secondary antibodies In order to visualize proteins on the membrane the antibodies are typically ...

  2. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...heavy-chain only antibodies in the serum of a camel and soon after found similar antibodies in llamas and...Comparison of a heavy-chain only antibody and single-domain antibody, under the commercial "Nanobody" ...conventional primary antibodies, and there are unique benefits to using sdAbs as secondary antibodies. sdAbs, however...single-domain antibodies without the need for a species that naturally produces heavy-chain only antibodies. (A)...heavy chain only antibodies) (B) Nanomice express hybrid heavy-chain only antibodies that are the source... were already on a quest for smaller forms of antibodies and had begun developing Fab fragments and scFvs...the potential for an even smaller, single-domain antibody fragment was exciting, and it wasn’t long until...

  3. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection...Addgene blog Antibodies 101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...single-fluorophore-conjugated antibodies. These methods can include labeling markers with multiple proteins/antibodies, targeting... to antibodies and 'staining' to refer to small molecule dyes. Indirect labeling While antibodies used...fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated antibodies may exist...perk is that one primary antibody can be bound by several secondary antibodies, increasing the fluorescence...selecting the primary antibody host species, as it has to be different from the other antibodies in your panel...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...multiclonal antibodies to increase overall signal from the primary antibody.  Pro tip! If your antibodies are ... your blocking, first check your antibody spec sheet. Some antibodies work better with specific blockers...make up a fresh batch.  Antibodies We always recommend either using an antibody validated for your protein...interest or validating an antibody yourself. But even with a validated antibody, it can still take time ...10,000 for primary antibodies). You’ll want to run a protein gradient to test the antibody at the recommended...outside the detectable range of that antibody dilution. Adjust the antibody dilution until your protein dilutions... biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range...

  5. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed...the first antibodies distributed by Addgene.  The plasmids used to generate these antibodies were based...express multiple subclasses of IgG antibody. Broad range secondary antibodies are commonly used in research...-Based Recombinant Antibodies  Plasmids 101: Secondary Nanobody Toolbox Antibodies 101: Introduction to... to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection References...distributed through NABOR are recombinant antibodies expressed from plasmids that have been deposited with Addgene...off of a large collection of mouse monoclonal antibodies (mAbs) that had already been thoroughly validated...

  6. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...resources on the Addgene blog Antibodies 101: Introduction to Antibodies New Antibody Animation ...bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question, like "what...what is an antibody?" or "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new ...new antibodies animation has all the information! Link in bio. #STEM #antibodies #molecularbiology #science...Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-...

  7. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...website and blog, including our antibody guide, antibody protocols, and Antibodies 101 blog series.  Stay tuned...-to-use recombinant antibodies. The first collection, a set of integrin antibodies, will be available ...s website this fall. IPI antibodies will come with access to the antibody sequences, in line with the...the rest of Addgene’s recombinant antibody collection, as well as access to IPI educational resources ...resources developed to support use of their antibody tools. Addgene also offers a growing collection of educational...

  8. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ...looking to use antibody-based applications in their research.  Like all our eBooks, the Antibodies 101 eBook...re very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody” quite useful...to find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody for Your Experiment...Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place to start...excited to share the very first edition of our Antibodies 101 eBook! The latest addition to our eBook collection...brings together all of our 101 blog posts on an antibody-specific topic, allowing you to easily access ...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...unique antibodies that bind discrete subunits or particular subunit pairs. Some of the antibodies in the...property in an antibody is extremely rare. While many function-blocking integrin antibodies exist, almost...immunohistochemistry. Table 1: IPI antibodies  Antibody Target Proteins Reactivity Isotype Source...research by distributing antibodies developed by IPI. Our integrin antibodies are the first deposit from...with Addgene to make our collection of integrin antibodies available through Addgene’s repository. The collection...anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either produced de novo using yeast display...

  10. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping... avoid retaining residual antibodies. If you have already applied antibodies and need to dry the membrane... primary antibody by re-blocking your membrane and re-incubating with your secondary antibody. This is...than start over, you can remove the detection antibodies from your membrane, allowing you to probe the...that you want to probe your blot with multiple antibodies. You’ll want to set up your blot with a polyvinylidene...will be removed from the membrane along with the antibodies, especially with more stringent stripping methods...step. Important: do not dry your membrane with antibodies on it if you plan to reprobe! Just as your sample...

  11. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...animation is here to help! "Antibodies 101: Antibodies in the Lab" shows how antibodies are used to study proteins... Addgene Addgene's Antibody Guide What is an Antibody? animation Addgene's Antibody 101 eBook    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...introduction to — or quick refresher on — these common antibody applications.  Thanks for watching, and we hope...

  12. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...protocol video!  Antibodies The most important part of your western is your antibodies, particularly your...your primary antibodies. We always recommend using an antibody validated for your target in your specific...polyclonal and monoclonal antibodies can work well for a western blot. Polyclonal antibodies, which bind to multiple...incubate their primary antibody overnight at 4 °C and their secondary antibody for 1–2 hrs at room temperature...to another or change primary antibodies as needed. However, more antibodies mean more optimization and ...require more antibody or loading sample, which may not be practical. Conjugated primary antibodies can be more...Before selecting one, check your antibody information. Many antibodies will have a recommended blocking...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ... from your antibody panel. They are made up of compensation beads and a conjugated antibody. Compensation...your sample. The isotype antibody will be virtually identical to the antibody for your marker of interest...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs. Spectral...Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection        ...Depending on the markers you are looking at and the antibodies you are using, you may need to include additional... synthetic beads that can bind to a conjugated antibody and beads that are unable to bind to them: like...The compensation beads can bind to any primary antibody you add. Single color controls are used to help...

  14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...recover good antibody binding by tweaking those parameters, but in some cases your antibody may just not... the Addgene blog: Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Epitope Availability...Availability Antibodies 101: Introduction to Antibodies Resources on Addgene.org Find recombinant antibodies...epitope of some targets, preventing your primary antibody from binding (Fig.1). The level of cross-linking...Fig. 1). So, while organic solvents may improve antibody binding for some targets, they can destroy it ... Figure 1: Choice of fixation method impacts antibody binding. Formaldehyde cross-links the protein ...BioRender.   Permeabilization What is the point?  Antibodies are large proteins that need a little help crossing...

  15. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    .../s12929-019-0592-z  Resources Antibodies 101: Introduction to Antibodies CRISPR in the Clinic   ...Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...cancer to autoimmune disorders. Antibodies administered as drugs are still immunogenic, meaning that they elicit...  ADAs and Adverse Immune Reactions Anti-drug antibodies (ADAs) are formed when the body recognizes a ...of immunogenicity can help evaluate risk of an antibody.  In silico prediction of adverse reactions In...cell epitopes are found in either the therapeutic antibody itself or as impurities in the formulation. Efforts...epitopes based on the amino acid sequence of the antibody.  NetMHCPan is one of the more common open-access...

  16. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...expression, measured using antibody conjugated fluorophores Fluorescent antibodies get around the challenges...staining a primary antibody binds to a target and a fluorochrome-conjugated secondary antibody binds to the ...are low as multiple secondary antibodies can bind to a primary antibody thus amplifying the signal.   ...should also include a secondary antibody control in which the primary antibody has been omitted from the staining... about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody plasmids...surface receptor are stained with a fluorescent antibody against the receptor and analyzed on a flow cytometer... binding to the protein in its native state.  Antibodies-based flow cytometry can use both direct and ...

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... Pooled library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl tRNA synthetases...Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based...recombinant antibody toolbox for aminoacyl tRNA synthetases By Kate Harten Recombinant antibodies targeting...these processes. To create the antibody toolbox, they screened antibody candidates using a synthetic human...whether the generated antibodies could bind these aaRSs. They found the antibodies were able to immunoprecipitate...sequencing. From Starr et al Cell., 2020. (B) Mapping antibody-escape mutations with the yeast display library...binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated...

  18. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...your samples with multiple antibodies, the fluorescent probes on the antibodies may have similar emission...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs. Spectral...this handy emission spectra chart to find your antibody's spectra). Your cytometer will therefore record...BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by different names depending on your flow ...

  19. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols  Resources on the...especially if downstream antibody detection is desired… and yes, we have an antibody for that!   Fig....of these tags also have commercially available antibodies, resins, beads, and other purification aids readily... to remove the cleaved tag from solution. MBP antibodies are available for detection and/or purification...purification of MBP-tagged proteins. The antibody is an alternative to amylose resin and also provides a visual...you are having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often ... We are here to help with vectors, protocols, antibodies, and more!   Resources and References   References...

  20. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    .... Name Our New Antibody Friend We need your help naming our latest mascot, an antibody joining us on ...following along on our journey to name our new antibody mascot, who's joining us in preparation for our...for Abi as they help us prepare for our upcoming antibody launch in late March!      *If you submitted ...Blugene and Aavery's newest friend, our Addgene antibody mascot, here to help us with our upcoming affinity...suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we...
Showing: 41 - 60 of 180 results