We narrowed to 236 results for: cloning

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Curious about what it's like to be a summer intern at Addgenie? Addgenie Intern Toj explains it all!

Aug. 8, 2024, 7:22 p.m.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Feb. 27, 2024, 2:15 p.m.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

March 8, 2017, 3:15 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

Aug. 10, 2021, 1:15 p.m.

Learn how DNA methylation may affect the activity of certain restriction endonucleases.

June 30, 2016, 2:30 p.m.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Oct. 11, 2018, 12:30 p.m.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Nov. 3, 2022, 12:15 p.m.

Check out the top ten blog posts on the Addgene blog from 2019. Topics include CRISPR, fluorescent protein, plasmids, and more.

Dec. 31, 2019, 2:26 p.m.

Learn about a useful technique to screen phage genomes for host binding proteins and discover new research avenues.

Sept. 29, 2016, 2:30 p.m.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Nov. 1, 2018, 12:35 p.m.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

Aug. 2, 2016, 2:30 p.m.

How much time does it take to clone a plasmid and how much does it cost? We do some back of the envelope math to try and figure it out!

March 23, 2023, 1:15 p.m.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Jan. 14, 2016, 3:30 p.m.

Addgene has reached 100,000 total reagents in the repository! The next milestone coming up is 100,000 and we'd like you to guess the date that happens (a contest).

April 8, 2021, 7:25 p.m.

Where did plasmids come from? Learn more about natural plasmids, the benefits they offer cells, and how scientists have adapted them into laboratory tools.

Oct. 8, 2024, 1:15 p.m.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

Perturb the activity of 17 different signaling pathways using the cancer pathways ORFs kit.

March 22, 2016, 2:30 p.m.

Learn how the 2017 Addgene-Seeding Labs Plasmid Grant Awardees will be using plasmids in their research.

July 18, 2017, 12:41 p.m.