We narrowed to 79 results for: donor

1. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

   Type

   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ...your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or ssDNA – see below...where the genetic changes occur in a test tube. The donor DNA substrate only requires ~50 nucleotides of homology...

2. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ...a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine and mOrange or for live cell imaging...

3. CASTing Off for New Shores in Human Genome Editing

   Type

   Blog Post

   Published

   June 10, 2025, 1:15 p.m.

   ...Type V-K systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate...

4. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ... by targeting start codons, splice acceptor and donor sites, and introducing premature stop codons.  ...

5. Hot Plasmids: FRET-Based Biosensors

   Type

   Blog Post

   Published

   May 6, 2014, 1:07 p.m.

   ... zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He says, these new colors ...

6. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   ...FRET) is the transfer of energy from an excited donor fluorophore to an acceptor fluorophore, which then...

7. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...actinin. When tension is high vs. low in the cell the donor and acceptor of a FRET pair on actinin will produce...

8. FlipGFP, a novel fluorescence protease reporter to study apoptosis

   Type

   Blog Post

   Published

   May 21, 2019, 12:10 p.m.

   ...quantify, due to small fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly...

9. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   Published

   June 27, 2019, 12:25 p.m.

   ...MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver a genetic ...

10. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...These assays can be designed to detect either a donor sequence integrated through homology-directed repair...

11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...The second vector is a homology directed repair donor plasmid with homology arms and a selection marker...

12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...possible without double-strand breaks (DSBs) or donor DNA templates.  Figure 1: An overview of... genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org...

13. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ... kb. This system is transfected into cells as a donor plasmid containing the transposon, along with a ...helper plasmid that expresses the transposase. The donor plasmid contains the gene(s) of interest flanked...

14. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...plain-language stories about Fred Hutch research, pieces for donors such as fundraising materials and progress reports...

15. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...sex pilus, a “bridge” of cytoplasm that allows a donor cell to transfer genetic material to a recipient...

16. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ... efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR to introduce...

17. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...Type Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from...

18. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...or to add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, ...

19. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...This makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer)... Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities...

20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for mammalian cell...