We narrowed to 80 results for: donor

..., sweat and tears. (Thanks to our healthy blood donor for keeping going while we worked out the cell capture...

...your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or ssDNA – see below...where the genetic changes occur in a test tube. The donor DNA substrate only requires ~50 nucleotides of homology...

...a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine and mOrange or for live cell imaging...

... by targeting start codons, splice acceptor and donor sites, and introducing premature stop codons.  ...

...Type V-K systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate...

... zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He says, these new colors ...

...These assays can be designed to detect either a donor sequence integrated through homology-directed repair...

...MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver a genetic ...

...quantify, due to small fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly...

...FRET) is the transfer of energy from an excited donor fluorophore to an acceptor fluorophore, which then...

...actinin. When tension is high vs. low in the cell the donor and acceptor of a FRET pair on actinin will produce...

...The second vector is a homology directed repair donor plasmid with homology arms and a selection marker...

...possible without double-strand breaks (DSBs) or donor DNA templates.  Figure 1: An overview of... genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org...

... kb. This system is transfected into cells as a donor plasmid containing the transposon, along with a ...helper plasmid that expresses the transposase. The donor plasmid contains the gene(s) of interest flanked...

...plain-language stories about Fred Hutch research, pieces for donors such as fundraising materials and progress reports...

...Type Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from...

... efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR to introduce...

...sex pilus, a “bridge” of cytoplasm that allows a donor cell to transfer genetic material to a recipient...

...or to add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, ...

...This makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer)... Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities...