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We narrowed to 179 results for: kit

Showing: 41 - 60 of 179 results

  1. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ...website crisprkit.org to request complete kits or find protocols and other resources. Find CRISPRkit plasmids...-engineered fat cells fight cancer Frugal CRISPR kit for classrooms Cell-surface proteomics for neurons...chromoproteins, the researchers developed a version of the kit that uses melanin production, allowing students to..., M., Lau, M. B., et al. (2024). A frugal CRISPR kit for equitable and accessible education in gene editing... out to address this limitation by developing CRISPRkit, an inexpensive system for CRISPR experiments ...easily quantify their results.  Figure 6: CRISPRkit uses a cell-free system to carry out transcription...explore cell metabolism. Plasmid components of CRISPRkit are now available through Addgene. And educators...

  2. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...which are enzyme-based mycoplasma detection kits. These kits detect mycoplasma enzymes that are not synthesized...specificity (Degeling, 2012). Bioluminescence assay kits are available from different biotech companies which...synthesized by eukaryotic cells. First, the kit components rupture the mycoplasma and then specific enzymes...

  3. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et...Resources on Addgene.org Find Additional Cloning Kits ...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds ... This selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each...plasmids are available in the Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References...

  5. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ..., plasmid kits are avaliable for less than than it would cost to buy each plasmid in the kit individually...This describes how the plasmid or other product (kit, pooled library, etc) will be sent to you. Most of...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... tools that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...biological systems” as they predict. The pOSIP plasmid kit can be found at Addgene, so what are you waiting ...constructs, while the Patron Lab MoClo Plant Parts kit contains modules specific for plant transformation... For further reading about Nicola Patron's MoClo kit and her plant synbio research, read the Addgene interview...annealing (SSA) assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces...additional information on using the Platinum Gate TALEN Kit, please see the Yamamoto lab’s protocol for TALEN...specifically to improve plant transgenesis. TheGreenGate kit available at Addgene includes six individual types...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized....1016/j.neuron.2024.08.019.  ONE-GO biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) ... of selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection...

  9. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...topoisomerase (2).  These days commercially available TOPO kits provide vectors or cloning arms with overhanging...covalently linked to topoisomerase. Vectors in these kits also often have the topoisomerase site inserted ...

  10. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...of the PCR reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready...

  11. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... the entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great ...great information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter...

  12. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...

  13. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... can be done in-lab using commercially available kits or ordered as synthesis modifications when purchasing...choose from and you can opt to do-it-yourself with a kit or purchase it commercially synthesized with a label...

  14. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish ...that can be activated to emit light with a simple kit, and is readily available on any number of secondary...

  16. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA. This technique works by...interested in detecting in your sample. When an ELISA kit is ordered, the wells are typically already coated...

  18. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...looking to conjugate antibodies yourself, there are kits available to make the process quick and easy. Before...antibody’s buffer is compatible with your conjugation kit, since many common antibody buffers and buffer additives...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...little more colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al...Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags...

  20. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta...
Showing: 41 - 60 of 179 results