We narrowed to 128 results for: pcr

Yeast genome modification plasmids have been developed for tagging yeast genes with fluorescent proteins, epitope tags, and more. Various systems are described to help you choose which to use for your research.

Nov. 19, 2013, 2:37 p.m.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

April 6, 2021, 12:30 p.m.

Ready to produce rAAV in your own lab? This 101 post can help!

July 16, 2024, 1:15 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Learn more about Addgene's NGS verification process for pooled libraries using real-life amplication data from the Brunello gRNA library.

Oct. 26, 2017, 1:59 p.m.

Addgene's new cloning grade DNA service allows you to start cloning right when you receive the plasmids from Addgene. No amplification and extractions necessary!

July 28, 2020, 1:15 p.m.

Learn about the best tips and tricks for modular cloning (MoClo) of plasmids!

July 8, 2025, 1:15 p.m.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Dec. 17, 2020, 1:30 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Find out what were Addgene's most popular blog posts in 2020!

Dec. 29, 2020, 2:15 p.m.

Learn how you can use planarian plasmids from the Sánchez lab to teach developmental biology techniques to new molecular biologists.

May 24, 2018, 1:20 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Learn how the Gradinaru Lab at Caltech developed AAV vectors to target the peripheral and central nervous systems.

Sept. 28, 2017, 2:01 p.m.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

Dec. 2, 2014, 7:23 p.m.

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Oct. 8, 2020, 1:15 p.m.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Dec. 15, 2016, 3:57 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

The first post in our summer science communication series.

June 9, 2022, 1:15 p.m.

RUBY-red is a new plant marker that uses a bright red color for easy identification of transgenic plants.

March 1, 2022, 2:15 p.m.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

June 28, 2016, 10:59 a.m.