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We narrowed to 772 results for: INA

Showing: 291 - 300 of 772 results

  1. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    .... We will continue to provide you with more fascinating, informative, and actionable content in the coming...genome editing.   2. Plasmids 101: Cre-lox Cre recombinase can be used to generate inversions, deletions...Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired of having...you’ve made the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation...

  2. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... embryonic stem cells, they found very high recombination percentages (78-79% after two rounds of transduction...application of iTOP Having tested iTOP using Cre-lox recombination, D’Astolfo et al. were eager to see if iTOP ...events. For these reasons, iTOP is best suited to binary systems in which transient protein expression has...long-lasting effect, such as Cre- or Cas9-mediated recombination. Both primary hESCs and immortalized cells treated...

  3. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...many cases, you won’t see any signs of DNA in your final tube after purification. How do you know if you ...your DNA prep and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally...method also gives an indicator of DNA or RNA contamination based on the presence of other bands or streaking...methods, this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis...

  4. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...I’ve always been fascinated by the human body and how it works. I used to stay up past my bedtime, poring...baby’s health but adamantly refused to get them vaccinated. A classmate told me that they never finish a...more likely to rate that study as untrustworthy, inaccurate, methodologically flawed, and biased. As a result...information gained from that research remains inaccessible, behind paywalls and in technical jargon that...

  5. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...annotate things as they come up in their minds. The final screen can also be saved and exported as a picture...miscommunication, a lack of leadership, and inappropriate tools or writing approaches can lead to frustration...frustration, delay of publication, or even the termination of a project.” Take brain breaks Meetings can...Wuchty S, Jones BF, Uzzi B (2007) The Increasing Dominance of Teams in Production of Knowledge. Science 316...

  6. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...projects, we offer classes, workshops, book clubs, seminars, and social events. Building a community science...community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering Project... not be pursued in an academic or industry lab. Mrina Nikrad, Ph.D., is an expert in microbial ecology...! Dennis Oleksyuk, Raphael Laurenceau, and Tami Pudina have initiated a soil microbiome project. This ...

  7. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...nearly all cell types in the body from otherwise terminally differentiated cells simply by expressing four... a community largely created due to Yamanaka’s seminal work. It didn't take very long to realize that...Hideyuki Okano presented ground-breaking work on spinal cord injuries; Jessica Young shared research on..., a half-brother who is a quadriplegic due to a spinal cord injury, and numerous family friends who had...

  8. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...recent years, there are many fluorescent protein combinations that can be imaged together, but this also means...microscope(s) you intend to use. An accurate determination of whether two fluorescent proteins can be separated...Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640 nm). In ...try several proteins in your system of interest. Finally, in this set of proteins the green and red proteins...

  9. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...backbone with a T7 promoter driving multiple combinations of WT, codon optimized, untagged, cleavage sites... sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include...found in the bacterial expression plasmids. Each combination is present in two sets of backbones which drive...expression system. Mammalian expression plasmids Finally a set of wild type and codon optimized, full length...

  10. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...antimicrobial.  Conjugates And HRP brings us handily to the final topic of this post, the conjugates, or signaling... a brief introduction to the wide, wild, and fascinating field of signaling molecules. Assay Type Westerns..., simple to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics: Antibodies...
Showing: 291 - 300 of 772 results