We narrowed to 783 results for: INA

...particles. A single viral particle is called a virion. Finally, viral vectors, which are used in the lab, are ...contrast to bacteria and eukaryotes, which use a binary replication process whereby one cell copies itself...engineering controls — changes made to the virus that eliminate or reduce the risk of infection — researchers ...genetically modified to either limit or completely eliminate their replicative ability. The remaining particle...

...next experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic...inputs are received by a cell. These so-called recombinase-based state machines could potentially be used...development. Read this post to learn more about recombinase-based state machines and how they function. Adapting...them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that...

...disturbing the tissue for imaging or collection could contaminate it. I have used it for selecting transformation...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al....untransformed seed from the same plant beginning to germinate. (Top right, second panel) Transformed seedlings... Biology Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy...

...the first vaccination and after both vaccinations. For the B.1.351 variant, a single vaccination didn’t ... SARS-CoV-2 variants coincide with increased vaccination efforts. So, how effective are the current vaccines...

...Ultragenyx Pharmaceutical in 2017. As one of the original employees at Dimension Therapeutics, she helped...interested in for a long time. “At an early age, I was fascinated by the impact that medicines can have on human...during graduate school at MIT. “I read some of the seminal papers on cell cycle discovery from Lee Hartwell...delivery. In 2018, he interviewed Addgene co-founder Melina Fan and former Associate Director of Viral Vectors...

...preparation approaches described previously require a preliminary double stranding step. The double stranding step... library preparation procedures. This finding eliminates the double-stranding step and reduces both the...confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an open-source...uses the VGS data to determine the percent of recombination present in Cre-dependent vectors and a script...

...sequences on a daily basis, many found these tools inadequate for planning, visualizing, and documenting their...elements such as promoters and transcription terminators, the boundaries are less obvious. We found that.... In an effort to be rigorous, we dug into the original literature, some of it decades old, to provide...mismatches, our algorithm could annotate a feature inaccurately. The best example is fluorescent proteins, which...

...electrical activity through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used...Chimeric channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV ...labeled neurons because background fluorescence from inactive neurons is reduced. 105322  AAV1  pGP-AAV-...densely-labeled neurons because background fluorescence from inactive neurons is reduced.  Additional resources...

.... We will continue to provide you with more fascinating, informative, and actionable content in the coming...genome editing.   2. Plasmids 101: Cre-lox Cre recombinase can be used to generate inversions, deletions...Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired of having...you’ve made the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation...

... embryonic stem cells, they found very high recombination percentages (78-79% after two rounds of transduction...application of iTOP Having tested iTOP using Cre-lox recombination, D’Astolfo et al. were eager to see if iTOP ...events. For these reasons, iTOP is best suited to binary systems in which transient protein expression has...long-lasting effect, such as Cre- or Cas9-mediated recombination. Both primary hESCs and immortalized cells treated...