We narrowed to 884 results for: ica

...To our surprise, we measured identical dissociation rates for catalytically inactive dCas9 molecules. We...altering its function). HDR is crucial for certain applications, for example, correcting the allele that causes...these editing reactions is low. Understanding the biological cause of this repair bias is a fascinating (and...(Richardson et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision...key scientific principle: that understanding a biological process in detail often suggests new strategies... dissociation rates and discovered that the catalytically active form of the nuclease dissociates very...Our in vitro results also demonstrated that catalytically inactive dCas9 produced a “bubble” structure...

... has been critical to the Broad’s success over the last 11 years and was certainly critical to drawing...background in history and specifically the history of RNAi left him a bit skeptical of the technology at the...cancer biology in Ed Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute...towards the Broad. In order to ask interesting biological questions, you need a tremendous amount of technology... in true collaboration with scientists asking biological questions. Doench loves that he still gets to...terms of determining what genes are involved in biological process X, you can do with CRISPR technology....with Addgene - he says he’s “known of Addgene basically since the beginning and [has] been using Addgene...

...ITRs serve as a type of origin of replication to initiate replication of the dsDNA virus. Viral packaging... endogenous E1 gene, making it replication deficient. This is typically supplied by the production cell...plasmid while the other plasmids with packaging or replication components are called helper plasmids. Below,...viral experiments is to deliver a genetic cargo, typically an expression construct, to cells/organisms. This... production, they can be helpful for certain applications and aid in ease of use. Our lentiviral guide...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...AAV are rep and cap. Rep is required for viral replication and also aids in infrequent genome integration...

...addition of a helper virus or plasmid allows it to replicate episomally, essentially remaining an extrachromosomal...extrachromosomal part of the host genome that can replicate and divide along with the endogenous DNA. AAV also...human gene therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory...cell type doesn’t always lend itself to direct application of virus. However, the cargo capacity of AAV ...gamma-retro viruses. This is large enough for many applications, but in some cases multiple AAVs (co-infection...that we’ve reviewed the common viruses used in biological research, you might have an idea which ones could...and AAV. If you’re wondering more about what applications these viruses are good for, we have plenty of...

...promising therapeutic applications; it can enhance therapeutic delivery across broader physical areas of the brain...driven by neurons. By studying how neurons are physically connected, we can begin to understand how the...Therefore, by simply looking at how neurons are physically connected we can begin to understand how the ...region (anterograde tracer, Fig. 1B). Tracers are typically delivered to a particular location and they either...neuronal signaling, or manipulating any other genetically controlled function. By using AAVrg in Cre transgenic...Cre-dependent AAV into Broca’s area of a mouse specifically expressing Cre in the hippocampus (Fig. 3). ... pigs (work you can find in AAV Data Hub!) The cortical pathways to the IC were well transduced by AAVrg...

...with biological protocols and revolutionary physical approaches. However, with each new technical achievement...aim to make microscopy as a tool accessible to practically everyone.     Fig. 1: Open source hardware...convinced that most microscopy techniques can be replicated with commercially available consumer-grade components...modify and optimize the code, find and fix security-critical bugs, and contribute to constant updates of the... OpenFlexure microscope or open-source modular optical toolbox openUC2 are trying to overcome this hurdle... tools has allowed the community to regularly replicate designs worldwide. Distributing hardware via globally...reproduction of the structure in a similar way to the biological protocol, and assembly designs based around the...

...your application a whole week before the deadline. The day of submission, review your application with... their dream.  To help students focus on the application process, and simplify access to academic resources...Scrutinize every opportunity prior to starting an application to assess your eligibility, interest, and career... detailed. Beyond the resume, a cover letter communicates your interest in a specific program, and institution...professors, and mentors. Ask for specific guidance on grammatical errors, sentence structures, and overall format...February represent peak season for internship applications. Thus these noble individuals are inundated ... with a stellar letter of support.        5. Application submission  Set a calendar reminder for every...

...limited understanding of plant biology is a significant technical obstacle to plant synthetic biology. We ...biotechnologists face is public perception of genetic modification (GM) and synthetic biology. The mistrust and...emerging engineering technology. Monoculture and chemical spraying, mainstays of modern agriculture, are... by developing a plant biotechnology product specifically for consumer sales. Flowers that change color...a new conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic biology In addition...cannot build what we do not understand, and the complicated and unique metabolic processes of plants are ...creative problem solving like the Howard Hughes Medical Institute have begun to pivot towards plant biotechnology...

...mice at a time. Because mice are more genetically and physiologically similar to humans than many other model... of mice as model organisms is that they are genetically tractable. Mice can be easily manipulated with...also relatively straightforward to manipulate genetically. Scientists can make a new line of flies in only...reasons above but because they actually share many biological properties and processes with our own cells. ...important experimental model in the study of neurological diseases, such as Parkinson’s disease (PD). ... throughout their lifetime. Thus any genetic modification, such as expression of fluorescent protein or...embryonic development to adulthood. This ability to genetically manipulate and visualize cells and tissues allows...

...immunofluorescence? Generally speaking, IF is an application in which antibodies that have been joined to ...molecules, knowing that where you see fluorescence indicates your target’s location. Some other terms you might...ICC or a tissue in the case of IHC. IF is used specifically when we visualize the antibodies using fluorescence...bind to a single primary, resulting in signal amplification. Another reason is cost effectiveness. Generating...are not exclusive to IF, but they can have a significant impact on the outcome of your IF experiment. ...controls to show that your antibodies are binding specifically. For positive controls, aim to use samples that...antibodies you choose for your experiment will have a significant impact on the protocol you use and the ultimate...