We narrowed to 848 results for: Abo;

...component of a biosensor is the thing that you care about within your cellular or animal system. This ‘thing...for expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We ...

...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for... that are otherwise cut off from the process.   About the author: Benedict Diederich received his PhD ...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used...

...blog to learn about making your own competent cells and our protocols page to learn about bacterial transformation...

...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg per mouse to get phenotypes...coverage. Please see this paper for more information about PHP.eB brain labeling at various doses.  As always...

...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...

...with limited packaging capacity. Still confused about the various tRNA expression strategies? No worries... Addgene. If you have any questions or thoughts about this technology, leave them in the comments below...

... extremely heterogeneous. There is consequently about a two-thirds chance of causing a frameshift mutation...favor accumulation of the latter products. As noted above, a single cycle of cleavage and accurate repair ...

...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...writing! Let us know if you have any other questions about writing and publishing your manuscript, or add your...

...talking about Click Chemistry since 2001, making use of robust reactions that work in just about any situation...

...power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

...Fluorescent 101 blog posts Read about chemogenetics vs. optogenetics Find more about the many types of protein...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

...in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning...of this system for enhancing cloning efficiency about 20 years ago and developed cloning vectors to harness...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...were observed binding telomeric DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9...

...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...