...your target base in the protospacer sequence. If you do not find a suitable ‘NGG’ PAM at your target genomic...

...tested with AYBEv3!). Although AYBE, AXBE, and ACBE do not approach the high product purity of CBEs and ...

...processes in different cell types. One great way to do this is to use CRY2-CIB1 clustering. Fuse either ...provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD...

..., phosphomimetic mutations on the activation loop do not fully activate MEK1. In fact, replacing just ...

...comparison. These next-generation sequencing techniques do not typically rely on Sanger sequencing but instead...

... by restriction enzyme cloning, you would have to do it in two steps, and a scar would likely remain between...

...default state. In contrast, Brainbow-3.1 and -3.2 do not display default fluorescence due to the addition...

...near optimal wavelengths (Fluc emits at 610 nm) and do not require external light sources. Unfortunately...

...systems listed above are orthogonal, meaning that they do not affect each other. For example, the GAL4 transcription...

...you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones? Here, I’...

...success, we find it is usually faster and easier to do the validation first.  AAV-mediated gene expression...

...recombinant clones are generated.  A simple way to do this is to express lambda red genes from a plasmid...

...μm) tissue sections and 3D cultures These samples do not necessarily require optical sectioning, but open...while also avoiding the toxic effects of high light doses to the cells. Thin static samples Ex: Fixed monolayers...out of focus light. However, they also continually dose the sample from top to bottom with excitation light...

...adaptive immune functions that other CRISPR types do, due to a lack of canonical CRISPR features (Pinilla-Redondo...

... a new or experienced reprogrammer? Which methods do you have experience using? Let us know about your...multiple transfection makes it difficult to control the dose of plasmid the cells receive over the whole reprogramming...

... significant medical interest, however efforts to do so have been hindered by a lack of tools, compounds...target soluble kinase domains, but these domains often do not express well in E. coli without their partner...cells. The mutant cells were treated with WNV at a dose and duration sufficient to kill all control cells...and not specific loci. To overcome this flaw, the Zoldos lab has developed CRISPR tools for epigenome editing...and their effects on gene regulation. Professor Zoldos and colleagues from the Department of the Biology...deletion efficiency was dependent upon 1) Cas9 and gRNA dose (both of which can be modulated using the different...

...Agudelo et al. prevent ouabain binding to ATP1A1 but do not compromise the ion pump’s function or cause cellular...

...authors the “Clonetegration”, is quick and easy to do. Clonetegration could become a “valuable technique...

...   Difference in neutralization after one dose and two doses of the mRNA vaccines While there have been.... Antibodies from individuals who received both doses of the Moderna or Pfizer vaccine could neutralize...from individuals who received either one or two doses of the Pfizer or Modern vaccine. This sera was used...emphasizing the importance of the second vaccine dose in mounting a robust immune response. The Balazs...

...present in the human population can affect drug and dose response. Selective serotonin reuptake inhibitors...are often ineffective or require several rounds of dose and drug adjustments. Unger ultimately hopes that... variants in vitro against different drugs and dosages.  Michal Bernach: Developing an artificial leaf...