We narrowed to 685 results for: Eng;

1. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...Leeson Multiplexed images in live cells remains a challenge due to multiple factors, including limitations...

2. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...number of constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody...

3. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...Cas9 (SpCas9) has been widely popular in genome engineering. For use in human cell lines, the Zhang lab codon-optimized...

4. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their target protein...

5. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...and your research community! We work to provide engaging and informative resources that answer questions...

6. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   Published

   Feb. 2, 2021, 2:15 p.m.

   ... while you make the ultimate case: why you? 7. Strength in numbers Encourage your close peers to come ...

7. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient, and, as...

8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ... Besides being fast and efficient, what other challenges does this system help to solve? Myers: Only a...

9. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   Published

   June 4, 2015, 1:03 p.m.

   ...practical use in the following way. Scientists engineered a multiple cloning site (MCS) into the α-peptide...

10. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    Published

    May 22, 2014, 4:02 p.m.

    ...the journal. Ask colleagues (preferably native English speakers) to proofread your manuscript and help...

11. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

    Published

    Feb. 4, 2014, 3:50 p.m.

    ...current position Understand your interpersonal challenges if they know some of the people you work with...

12. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...genomes of mammalian or other cells. TALENs are engineered proteins that comprise a DNA binding domain and...

13. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

14. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued...

15. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    Published

    April 20, 2023, 4 p.m.

    ...gives your entire audience a chance to comfortably engage with you. If you can give a seminar, lead a journal...

16. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

17. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...promoters Tetracycline-dependent promoters are engineered by placing tet response elements upstream of ...

18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...

19. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...