We narrowed to 784 results for: IRE;

... and red fluorescence reporting, respectively. Inspired by recent improvements on another GPCR-based reporter...pooled TCR assembly method. Step 1 inserts oligos of paired CDR3β-α sequences into TRBV-TRAC vectors to generate...

...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,..., M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity...

...without limitations and well-executed experiments require some consideration of FP properties. It is important...detected in microscopy cannot be interpreted as direct molecular interaction without further investigation...

.... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using...emission and absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers...

...used to push your research in new and exciting directions. Drosophila are very easy to manipulate genetically...fly-based experimental systems. Some resources will direct you to the latest information about gene function...

...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...in the E. coli lab strain DH5a, but others may require strains such as ccdB survival (for Gateway plasmids...

...poster: those that want you to walk them through the entire poster, or those that want to look through themselves...handle on your nametag for the tweep up (and for the entire conference, in general). After the conference Review...

..., the drive could eventually spread through the entire wild population with unpredictable ecological effects...ecological, and reproductive confinement typically require very little effort to implement (depending on the...

...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...

...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

...think of as an operating system for engineering an entire cell. Typically when people use plasmids, they ...much easier. So you could imagine engineering the entirety of a cell. That's a big long-term technical challenge...

... just be sure to check that your RNase has not expired (or been forgotten).    Improving read accuracy...purification protocol. Are any of your materials expired? Did you pipette carefully? Remember to discard...

...choose, you may not be able to elute the antibodies directly into your preferred buffer. If that’s the case...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

...impacted the experiments we wanted to perform. It required a great deal of time and effort to test and optimize...J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives...

...availability following cross-linking fixation; they require an additional step generally referred to as Antigen...retrieval here because it’s not quite as ubiquitously required for IF as fixing and permeabilizing.  References...

...modeling isn’t up your alley, feel free to skip directly to the results. To start modeling our data, we...chemistry and chemical biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the ...

...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets endogenous...

... that if an essential gene plays a role in the desired screening phenotype, it may be missed because it's...transcriptional machinery to dCas9 and its RNA directed binding site. These methods include the fusion...

...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector...

... PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular...