We narrowed to 776 results for: IRE;

.... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using...emission and absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers...

...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,..., M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity...

..., the drive could eventually spread through the entire wild population with unpredictable ecological effects...ecological, and reproductive confinement typically require very little effort to implement (depending on the...

...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...

...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

...think of as an operating system for engineering an entire cell. Typically when people use plasmids, they ...much easier. So you could imagine engineering the entirety of a cell. That's a big long-term technical challenge...

...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets endogenous...

...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector...

... PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular...

... that if an essential gene plays a role in the desired screening phenotype, it may be missed because it's...transcriptional machinery to dCas9 and its RNA directed binding site. These methods include the fusion...

...modeling isn’t up your alley, feel free to skip directly to the results. To start modeling our data, we...chemistry and chemical biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the ...

...impacted the experiments we wanted to perform. It required a great deal of time and effort to test and optimize...J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives...

...availability following cross-linking fixation; they require an additional step generally referred to as Antigen...retrieval here because it’s not quite as ubiquitously required for IF as fixing and permeabilizing.  References...

... just be sure to check that your RNase has not expired (or been forgotten).    Improving read accuracy...purification protocol. Are any of your materials expired? Did you pipette carefully? Remember to discard...

...choose, you may not be able to elute the antibodies directly into your preferred buffer. If that’s the case...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

...affinity purification, in 2009. However, iChIP requires insertion of recognition sequences of an exogenous...into the target region. However, it struck me that direct use of such engineered DNA-binding molecules even...

...all of these platforms were developing, Google acquired Youtube and has been growing its online video ..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...

...They enjoy the fact that each new challenge will require knowledge of new topics and that they will switch...analyses skills were applicable to my cases. I can directly use my understanding of the molecular behaviors...

...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...course of infection. These binding events can be impaired by the electrostatic repulsion of the negatively...

...process of onboarding a new employee who's coming directly from grad school. The transition has been a bit...thought they would be working in a lab for their entire career. Yet even success in academia means you’...