We narrowed to 821 results for: LAS

...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

...double-membraned structures containing undigested cytoplasmic contents. Autophagosomes that have fused with... example of what fluorescence looks like for cytoplasmic mitochondrial Rosella with and without autophagy... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors...

...Flash quiz! What pops into your head when you hear the phrase “action potential?” Firing neurons are ...through gap junction proteins which connect the cytoplasm of two cells) can also affect patterning and in...GEVI features increased brightness and improved plasma membrane specificity. Stable Step Function Opsin...

...   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference from Cas9.... Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015...More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR Guide  ...

...to antibodies. Instead, they are derived from a class of binding proteins known as ankyrin repeat proteins...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...available from AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018)....

... give as a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels... assignment was clear: present a paper to our classmates so they could understand it. When my mind went...incidents above–and the dumpster noises were just last week!  Nerves It’s completely normal to be anxious...

... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

...Chris Voigt for speaking with us! Find the Plasmids: Plasmids for multi-input CRISPR/Cas9 genetic circuits... required to get the function that you want. The last few years, we’ve really been making tons of parts...

...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...

...the Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I...Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she took her...interest in data science,” which she’s taking some classes on and hoping to pursue. The choice to not go to...

...drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that oxidize 5-methylcytosine...achieved by dCas9 alone, without TET (or any demethylase) fusion. This may seem counterintuitive: how ...achieved using lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple...

... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...raising the threshold for signaling. The two main classes of receptors currently used in chemogenetics are...viral preps for your research Find chemogenetics plasmids for your research ...

...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...the full amount of time, every time. Imaging The last step in a western blot tends to be the most straightforward...

...large-scale prime editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab...sciadv.aax9249 Koeppel, J., Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante....1038/s41587-022-01613-7 Mathis, N., Allam, A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L...

...Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis...amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence of an insert...

...because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design ...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also ...Resources on Addgene.org Find fluorescent protein plasmids and resources Visit our Molecular Biology Reference...

...neurogenesis, neuronal survival, and synaptic plasticity. Several studies have found reduced BDNF levels...prefrontal cortex Drd1 neurons produces rapid and long-lasting antidepressant effects." Nature communications ...Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic system in addiction....

...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...collection Read our molecular biology reference Find plasmids for your research ...

...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...Cichlids are 2,000 fish species that evolved in the last 10,000 years (which is considered recent). They ...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They...

...profile problems surrounding cell culture over the last few decades some pharmacologists have tended to ... information in your lab book and on the actual flasks of cells in the incubator). This means any other...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...