Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 809 results for: LAS

Showing: 581 - 600 of 809 results

  1. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...raising the threshold for signaling. The two main classes of receptors currently used in chemogenetics are...viral preps for your research Find chemogenetics plasmids for your research ...

  2. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...the full amount of time, every time. Imaging The last step in a western blot tends to be the most straightforward...

  3. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis...amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence of an insert...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...large-scale prime editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab...sciadv.aax9249 Koeppel, J., Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante....1038/s41587-022-01613-7 Mathis, N., Allam, A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L...

  6. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...neurogenesis, neuronal survival, and synaptic plasticity. Several studies have found reduced BDNF levels...prefrontal cortex Drd1 neurons produces rapid and long-lasting antidepressant effects." Nature communications ...Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic system in addiction....

  8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...Cichlids are 2,000 fish species that evolved in the last 10,000 years (which is considered recent). They ...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...profile problems surrounding cell culture over the last few decades some pharmacologists have tended to ... information in your lab book and on the actual flasks of cells in the incubator). This means any other...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

  10. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...-time, and have received a research grant in the last five years as polled in the American Association... funding for science has been decreasing for the last five years and the first federal budget request ...March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student edition...

  11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers...more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-19 research Resources on ...in our CRISPR guide Browse our entire COVID-19 plasmid collection ...

  12. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...cellular compartments such as the mitochondria, cytoplasm and nucleus.  We hope that this blog has provided...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...experiments? In this post, we will discuss some broad classes of optogenetic tools, and what factors to consider...using optogenetic tools Once you’ve selected the class of tool appropriate for your experiment, you’ll ... https://doi.org/10.1038/nmeth.1808 Nagel, G., Szellas, T., Huhn, W., Kateriya, S., Adeishvili, N., Berthold...

  14. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward the cell...be easily performed too. While I implied that axoplasmic transport of AAVs is a potential problem in many...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  15. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... a few gRNAs suggested to be effective by world class CRISPR labs.  Check Out This Video to See How Green...particular, one slide of the presentation made a lasting impression on me. The slide showed a complex signaling...Find CRISPR Screening Libraries Browse All CRISPR Plasmids ...

  16. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ... models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs are the photoconvertible ... (level of zoom, intensity, possible excitation lasers) it is also important to optimize your activation...Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting...

  17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...mitochondria, and different dyes can result in varying cytoplasmic background signals when used with the same protein...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...More resources from Addgene Fluorescent protein plasmids and resourcesA practical approach to choosing ...

  18. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... making fiber optic cables only to hook up your lasers and not have the proper controls or a proper behavioral...surgeries. Identify coordinates through a rodent brain atlas or research papers and practice with die injection...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ...

  19. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...2021). TGFβ1 induces resistance of human lung myofibroblasts to cell death via down-regulation of TRPA1 ...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...about that collection. Table 1: Systemic capsid plasmids available from Addgene Capsid Published ...re interested in. Then request your capsid (or plasmids) and let the real work begin — validation, validation...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...
Showing: 581 - 600 of 809 results